caa a22 4500 465774059 CHVBK 20180323111937.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00493472 doi (NATIONALLICENCE)springer-10.1007/BF00493472 Effects of cytotoxic prostaglandin, Δ12PGJ2 on protein synthesis and cytoskeleton in transformed epidermal cells in culture [Elektronische Daten] [K. Ikai, M. Fukushima] Summary: Cyclopentenone prostaglandins (PGs) such as Δ12-PGJ2 and PGA are potent inducers of growth inhibition in a variety of cultured cells, including epidermal cells. These PGs are actively transported into cells by a specific carrier on cell membrane and accumulate in cell nuclei with binding to nuclear protein. To clarify the mechanism of cytotoxicity of these PGs in epidermal cells, we examined the effects of Δ12-PGJ2 on protein synthesis and cytoskeleton in the PAM 212 transformed mouse epidermal cell line. Cycloheximide at 1 Μg/ml culture medium exhibited a protective effect on cell growth inhibition of PAM 212 cells by Δ12-PGJ2. The analysis of cell lysate protein patterns by SDS-polyacrylamide gel electrophoresis revealed that 12-h incubation with Δ12-PGJ2 increased the amount of 70 kD protein in PAM 212 cells. The amount of 70 kD protein in Δ12-PGJ2-treated cells was markedly decreased by cotreatment with cycloheximide. This 70 kD protein was also induced in PAM 212 cells with treatment at 43‡C for 90 min, indicating that this synthesized protein belongs to the heat shock protein. The addition of Δ12-PGJ2 to confluent PAM 212 cells resulted in the disappearance of action filament, as visualized by fluorescent labeled phallacidine, but in contrast, keratin filament appeared to be intact during 12-h incubation with Δ12-PGJ2 at a concentration of 5 Μg/ml culture medium. These results suggest that the cytotoxicity of cyclopentenone PGs is at least in part due to induction of the synthesis of some protein(s), probably one of the heat shock proteins, and the damage to the actin filament in transformed cultured epidermal cells. Springer-Verlag, 1990 Cytotoxicity nationallicence PAM 212 cells nationallicence Δ12-PGJ2 nationallicence Heat shock protein nationallicence Cytoskeleton nationallicence Ikai K. Department of Dermatology, Kyoto University Faculty of Medicine, Sakyo-ku, 606, Kyoto, Japan aut Fukushima M. Department of Internal Medicine and Laboratory of Chemotherapy, Aichi Cancer Center, 464, Nagoya, Japan aut Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 131-134 0340-3696 282:2<131 1990 282 403 https://doi.org/10.1007/BF00493472 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00493472 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ikai K. Department of Dermatology, Kyoto University Faculty of Medicine, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Fukushima M. Department of Internal Medicine and Laboratory of Chemotherapy, Aichi Cancer Center, 464, Nagoya, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 131-134 0340-3696 282:2<131 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer