caa a22 4500 465774156 CHVBK 20180323111937.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00412739 doi (NATIONALLICENCE)springer-10.1007/BF00412739 Cultivation of keratinocytes derived from epidermal explants of sheep skin and the roles of growth factors in the regulation of proliferation [Elektronische Daten] [P. Pisansarakit, D. du Cros, G. Moore] Summary: Procedures to promote the growth of primary cultures of keratinocytes derived from sheep epidermis through several passages are described. Rapid epithelial outgrowth was obtained from explants of epidermis isolated from trypsinised inguinal skin biopsy specimens. Following initiation and attachment, cells displayed the polygonal morphology typical of keratinocytes in culture and survived a number of passages before terminally differentiating and sloughing from the surface of the culture vessel. Proliferation occurred in the absence of a feeder layer and was attained in a medium supplemented with foetal bovine serum and hydrocortisone or cholera toxin. Growth was stimulated by the addition of epidermal growth factor or fibroblast growth factor (FGF) to the culture medium. The detection of basic-FGF immunoreactivity in Western immunoblats of extracts of fresh tissues suggests a role for this factor in autocrine or paracrine growth regulation of skin cell populations in vivo. Springer-Verlag, 1990 Keratinocytes nationallicence Sheep culture nationallicence Growth factors nationallicence Pisansarakit P. Division of Animal Production, CSIRO, P.O.Box 239, 2148, Blacktown, NSW, Australia aut du Cros D. Division of Animal Production, CSIRO, P.O.Box 239, 2148, Blacktown, NSW, Australia aut Moore G. Division of Animal Production, CSIRO, P.O.Box 239, 2148, Blacktown, NSW, Australia aut Archives of Dermatological Research Springer-Verlag 281/8(1990-01-01), 530-535 0340-3696 281:8<530 1990 281 403 https://doi.org/10.1007/BF00412739 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00412739 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pisansarakit P. Division of Animal Production, CSIRO, P.O.Box 239, 2148, Blacktown, NSW, Australia aut NATIONALLICENCE 700 1- du Cros D. Division of Animal Production, CSIRO, P.O.Box 239, 2148, Blacktown, NSW, Australia aut NATIONALLICENCE 700 1- Moore G. Division of Animal Production, CSIRO, P.O.Box 239, 2148, Blacktown, NSW, Australia aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 281/8(1990-01-01), 530-535 0340-3696 281:8<530 1990 281 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer