caa a22 4500 465777015 CHVBK 20180323111944.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF02764295 doi (NATIONALLICENCE)springer-10.1007/BF02764295 Metabolic analysis of reepithelializing rabbit cornea using phosphorus-31 nuclear magnetic resonance spectroscopy [Elektronische Daten] [Ken Hayashi, Hong-Ming Cheng, Masayuki Iwasaki, Hua Xiong, Kenneth Kenyon] To investigate metabolic differences between the central and peripheral cornea the latter including the limbal area, corneas were dissected and examined using phosphorus-31 (31P) nuclear magnetic resonance spectroscopy. Since most31P signals originate from the epithelium,31P spectra of the cornea primarily represent the metabolic state of the epithelium. The spectra of the peripheral cornea showed all phosphorus resonances detected in the whole cornea; in contrast, the central cornea showed no phosphocreatine and glycerophosrylethanolamine, and only low levels of ATP. These results indicate that there is a higher metabolic activity in the peripheral epithelium, especially in the limbal area, than in the central epithelium. To evaluate the metabolic state of corneal epithelium during regeneration, we also examined corneas reepithelializing after 7 mm of central epithelial tissue had been removed by mechanical scraping. Rabbits were killed 24 and 48 h after scraping. The reepithelializing corneas clearly showed an increase in ATP, phosphocreatine, and sugar phosphates with time, although phosphorylcholine remained depressed. These findings suggest that the reepithelializing cornea has an elevated level of energy production and that it may have reached a higher steady state, thereby indicating accelerated metabolism of the epithelium during regeneration. Springer-Verlag, 1990 Hayashi Ken Massachusetts Eye and Ear Infirmary, USA aut Cheng Hong-Ming Howe Laboratory of Ophthalmology, Harvard Medical School, USA aut Iwasaki Masayuki Department of Ophthalmology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812, Fukuoka, Japan aut Xiong Hua Howe Laboratory of Ophthalmology, Harvard Medical School, USA aut Kenyon Kenneth Massachusetts Eye and Ear Infirmary, USA aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/1(1990-01-01), 73-77 0721-832X 228:1<73 1990 228 417 https://doi.org/10.1007/BF02764295 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02764295 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hayashi Ken Massachusetts Eye and Ear Infirmary, USA aut NATIONALLICENCE 700 1- Cheng Hong-Ming Howe Laboratory of Ophthalmology, Harvard Medical School, USA aut NATIONALLICENCE 700 1- Iwasaki Masayuki Department of Ophthalmology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Xiong Hua Howe Laboratory of Ophthalmology, Harvard Medical School, USA aut NATIONALLICENCE 700 1- Kenyon Kenneth Massachusetts Eye and Ear Infirmary, USA aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/1(1990-01-01), 73-77 0721-832X 228:1<73 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer