caa a22 4500 465777279 CHVBK 20180323111945.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF02764296 doi (NATIONALLICENCE)springer-10.1007/BF02764296 Effective methods for the investigation of human tear film proteins and lipids [Elektronische Daten] [Gregor Wollensak, Erich Mur, Annemarie Mayr, Gottfried Baier, Wolfgang Göttinger, Georg Stöffler] The investigation of human tear film proteins and lipids is of value for the elucidation of contact lens incompatibilities, tear film instabilities, dry eye syndrome and various other eye diseases. Improved efficient methods for the investigation of human tear film proteins and lipids are presented in this paper. Tear proteins were examined by ultrathin sodium dodecyl sulfate-polyacrylamide gel electrophoresis, celluloacetate gel, isoelectric focusing, and high-performance liquid chromatography. The methods differ in sensitivity, resolution, convenience and reproducibility. Tear lipids were examined by high-performance thin-layer chromatography, and good separation into the major lipid classes was achieved. With this method it is possible to examine the lipids present in tears of an individual subject and not just in pools made up from the tears of several persons. Springer-Verlag, 1990 Wollensak Gregor Institut für Mikrobiologie, Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6020, Innsbruck, Austria aut Mur Erich Institut für Mikrobiologie, Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6020, Innsbruck, Austria aut Mayr Annemarie Institut für Mikrobiologie, Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6020, Innsbruck, Austria aut Baier Gottfried Institut für Mikrobiologie, Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6020, Innsbruck, Austria aut Göttinger Wolfgang Universitäts-Augenklinik, Anichstrasse 35, A-6020, Innsbruck, Austria aut Stöffler Georg Institut für Mikrobiologie, Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6020, Innsbruck, Austria aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/1(1990-01-01), 78-82 0721-832X 228:1<78 1990 228 417 https://doi.org/10.1007/BF02764296 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02764296 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wollensak Gregor Institut für Mikrobiologie, Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Mur Erich Institut für Mikrobiologie, Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Mayr Annemarie Institut für Mikrobiologie, Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Baier Gottfried Institut für Mikrobiologie, Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Göttinger Wolfgang Universitäts-Augenklinik, Anichstrasse 35, A-6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Stöffler Georg Institut für Mikrobiologie, Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6020, Innsbruck, Austria aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/1(1990-01-01), 78-82 0721-832X 228:1<78 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer