caa a22 4500 465777317 CHVBK 20180323111945.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF02764298 doi (NATIONALLICENCE)springer-10.1007/BF02764298 HLA DR and DQ expression on human retinal pigment epithelial cells in vitro [Elektronische Daten] [Christophe Baudouin, Danielle Fredj-Reygrobellet, Didier Jambou, Pierre Gastaud, Philippe Lapalus] In a previous immunohistopathological study, we demonstrated a deviant expression of class II antigens on the uveal pigment epithelial cells of patients with proliferative diabetic retinopathy. The mechanisms triggering this abnormal expression by epithelial cells are not well known, and we tried to induce this phenomenon on primary cultures of human retinal pigment epithelial (RPE) cells. Confluent RPE-cell monolayers were supplemented with several biological or chemical reagents [recombinant interferon gamma, phytohemaglutinin A-P (PHA-P), phorbolmyristate acetate (PMA), recombinant interleukin-2, fibroblast growth factor (FGF), Insulin], to investigate their ability to induce HLA DR and DQ expression. On days 1, 3 and 5 after stimulation, the cells were incubated with monoclonal antibodies directed against human class II antigens: all reagents used failed to induce class II antigen expression. However, on day 7, we demonstrated the presence of numerous positive HLA DR and HLA DQ cells stimulated by gamma interferon, the percentages being closely related to the dose of this lymphokine. These findings, together with those of other investigators and our previous work on uveal pigmented epithelial cells in diabetic patients, may shed light on the exact implication of RPE in many poorly documented ocular diseases. Springer-Verlag, 1990 Baudouin Christophe Department of Ophthalmology, Saint Roch Hospital, Nice, France aut Fredj-Reygrobellet Danielle Laboratory of Pharmacology, School of Medicine, Avenue de Vallombrose, F-06034, Nice Cedex, France aut Jambou Didier Laboratory of Pharmacology, School of Medicine, Avenue de Vallombrose, F-06034, Nice Cedex, France aut Gastaud Pierre Department of Ophthalmology, Saint Roch Hospital, Nice, France aut Lapalus Philippe Laboratory of Pharmacology, School of Medicine, Avenue de Vallombrose, F-06034, Nice Cedex, France aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/1(1990-01-01), 86-89 0721-832X 228:1<86 1990 228 417 https://doi.org/10.1007/BF02764298 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02764298 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baudouin Christophe Department of Ophthalmology, Saint Roch Hospital, Nice, France aut NATIONALLICENCE 700 1- Fredj-Reygrobellet Danielle Laboratory of Pharmacology, School of Medicine, Avenue de Vallombrose, F-06034, Nice Cedex, France aut NATIONALLICENCE 700 1- Jambou Didier Laboratory of Pharmacology, School of Medicine, Avenue de Vallombrose, F-06034, Nice Cedex, France aut NATIONALLICENCE 700 1- Gastaud Pierre Department of Ophthalmology, Saint Roch Hospital, Nice, France aut NATIONALLICENCE 700 1- Lapalus Philippe Laboratory of Pharmacology, School of Medicine, Avenue de Vallombrose, F-06034, Nice Cedex, France aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/1(1990-01-01), 86-89 0721-832X 228:1<86 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer