caa a22 4500 465777333 CHVBK 20180323111945.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF02764313 doi (NATIONALLICENCE)springer-10.1007/BF02764313 Thrombin stimulates the proliferation of human retinal glial cells [Elektronische Daten] [Donald Puro, Tomiya Mano, Chi-Chao Chan, Masakatsu Fukuda, Hiroyuki Shimada] Retinal glial cells may play a role in most of the proliferative retinopathies. Although glial cell proliferation is a frequent event in retinal pathobiology, no specific mitogens for human retinal glial cells are known. Using cultured retinal glial cells obtained from postmortem adult human eyes, we found that thrombin stimulates glial cell proliferation in a dose-dependent manner with a half-maximal concentration of 100 ng/ml (0.4 U/ml). Thus, thrombin may be a plasma-derived mitogen capable of stimulating retinal glial cells to proliferate when there is a breakdown of the blood-retinal barrier. We also observed that this proliferative response of retinal glia requires more than 6 h of continuous exposure to thrombin. This finding suggests that a thorough wash-out of a thrombin-containing infusate and/or the rapid inactivation of this molecule would prevent thrombin from exacerbating a proliferative disorder of the retina. Springer-Verlag, 1990 Puro Donald Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, P.O. Box 016880, 33101, Miami, FL, USA aut Mano Tomiya Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, P.O. Box 016880, 33101, Miami, FL, USA aut Chan Chi-Chao National Eye Institute, National Institutes of Health, Bethesda, Md, USA aut Fukuda Masakatsu Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, P.O. Box 016880, 33101, Miami, FL, USA aut Shimada Hiroyuki Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, P.O. Box 016880, 33101, Miami, FL, USA aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/1(1990-01-01), 169-173 0721-832X 228:1<169 1990 228 417 https://doi.org/10.1007/BF02764313 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02764313 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Puro Donald Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, P.O. Box 016880, 33101, Miami, FL, USA aut NATIONALLICENCE 700 1- Mano Tomiya Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, P.O. Box 016880, 33101, Miami, FL, USA aut NATIONALLICENCE 700 1- Chan Chi-Chao National Eye Institute, National Institutes of Health, Bethesda, Md, USA aut NATIONALLICENCE 700 1- Fukuda Masakatsu Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, P.O. Box 016880, 33101, Miami, FL, USA aut NATIONALLICENCE 700 1- Shimada Hiroyuki Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, P.O. Box 016880, 33101, Miami, FL, USA aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/1(1990-01-01), 169-173 0721-832X 228:1<169 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 465777333 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer