caa a22 4500 465777341 CHVBK 20180323111945.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF02764290 doi (NATIONALLICENCE)springer-10.1007/BF02764290 Response of Müller cells following experimental lensectomy-vitrectomy [Elektronische Daten] [Yusuf Durlu, Sei-ichi Ishiguro, Akiko Yoshida, Takezo Mito, Makio Tsuchiya, Makoto Tamai] We used morphological, biochemical and immunohistochemical methods to assess the response of Müller cells after experimental lensectomy-vitrectomy in rabbits. We observed widened intercellular spaces between the Müller cells and nerve fibers of ganglion cells, and increased electron opacity in the Müller cells of eyes injected with silicone oil. No apparent morphological changes were detected in the Müller cells of air-injected eyes. The specific and total activities of Müller cell-marker enzymes (glucose 6-phosphatase and glutamine synthetase) showed an initial increase, followed by a decrease. Glial fibrillary acidic protein immunoreactivity was not found in the Müller cells of the normal rabbit retina but was exhibited after surgery. Our results showed that markers of Müller cells associated with glycogenolysis and/or gluconeogenesis, glutamate-glutamine cycle and cytoskeletal protein metabolism were affected by the experimental lensectomy-vitrectomy. Springer-Verlag, 1990 Durlu Yusuf Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut Ishiguro Sei-ichi Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut Yoshida Akiko Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut Mito Takezo Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut Tsuchiya Makio Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut Tamai Makoto Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/1(1990-01-01), 44-48 0721-832X 228:1<44 1990 228 417 https://doi.org/10.1007/BF02764290 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02764290 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Durlu Yusuf Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut NATIONALLICENCE 700 1- Ishiguro Sei-ichi Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut NATIONALLICENCE 700 1- Yoshida Akiko Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut NATIONALLICENCE 700 1- Mito Takezo Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut NATIONALLICENCE 700 1- Tsuchiya Makio Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut NATIONALLICENCE 700 1- Tamai Makoto Department of Ophthalmology, Tohoku University School of Medicine, 1-1 Seiryo-machi, 980, Sendai, Miyagi, Japan aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/1(1990-01-01), 44-48 0721-832X 228:1<44 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer