caa a22 4500 465777368 CHVBK 20180323111945.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00918487 doi (NATIONALLICENCE)springer-10.1007/BF00918487 Measurements of antifungal levels in corneal tissue a simplified bioassay for amphotericin B [Elektronische Daten] [Trilby Williams, Denis O'Day, W. Head, Richard Robinson] Measuring precise antifungal levels in the cornea with broth-dilution bioassays is difficult, as standard techniques involving visual determination of endpoints are hindered by corneal debris. To increase the precision of the measurement, we modified the sample preparation for bioassay of rabbit corneas treated with subconjunctival Amphotericin B. Endpoint determination and variance were compared for a freshly thawed corneal suspension and the supernatant after 24 h equilibration; bioassay of the corneal suspension after 24 h equilibration served as an additional control. All endpoints were read visually in a masked fashion and were verified by culture. The three methods gave comparable endpoint values with equivalent degrees of variance. Amphotericin B levels were consistent by both visual and culture determination; however, endpoints were clearly visible and easier to read for the supernatant. Visual determination of the endpoints for the supernatant following 24 h equilibration simplified and ensured the precision of the bioassay technique. Springer-Verlag, 1990 Williams Trilby Department of Ophthalmology, Vanderbilt University School of Medicine, Nashville, Tenn, USA aut O'Day Denis Department of Ophthalmology, Vanderbilt University School of Medicine, Nashville, Tenn, USA aut Head W. Department of Ophthalmology, Vanderbilt University School of Medicine, Nashville, Tenn, USA aut Robinson Richard Department of Ophthalmology, Vanderbilt University School of Medicine, Nashville, Tenn, USA aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 538-540 0721-832X 228:6<538 1990 228 417 https://doi.org/10.1007/BF00918487 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00918487 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Williams Trilby Department of Ophthalmology, Vanderbilt University School of Medicine, Nashville, Tenn, USA aut NATIONALLICENCE 700 1- O'Day Denis Department of Ophthalmology, Vanderbilt University School of Medicine, Nashville, Tenn, USA aut NATIONALLICENCE 700 1- Head W. Department of Ophthalmology, Vanderbilt University School of Medicine, Nashville, Tenn, USA aut NATIONALLICENCE 700 1- Robinson Richard Department of Ophthalmology, Vanderbilt University School of Medicine, Nashville, Tenn, USA aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 538-540 0721-832X 228:6<538 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer