caa a22 4500 465777392 CHVBK 20180323111946.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00918488 doi (NATIONALLICENCE)springer-10.1007/BF00918488 Alterations in rabbit vitreal fine structure following C3F8 injection [Elektronische Daten] [Barbara Panessa-Warren, James Maisel, John Warren] This study examines the morphological and histochemical changes in the cortical vitreous of 36 rabbit eyes following C3F8 intravitreal gas injection. Eyes were examined by light microscopy (LM) using a modified cryofixation and cryosectioning technique that prevented the loss of soluble tissue moieties and permitted collagen and proteoglycan histochemistry as well as enzyme digestion with hyaluronidase. LM and scanning electron microscopy (SEM) of cryosectioned normal eyes revealed an elaborate fibrillar matrix extending 100-190 gin from the basal lamina of the retina into the vitreous proper, which seemed to be composed of collagen fibrils intimately associated or wrapped in proteoglycan. Following the full expansion of the C3F8 gas bubble in the vitreous, the cortical fibrillar meshwork was absent from the retinal surface and a dense, collagenous material accumulated in the anterior vitreous, especially between the ciliary processes and over the posterior face of the lens. At 41 days postinjection, the fibrillar matrix was reforming and the vitreal cavity was filled with fluid and numerous fibrillar-mucinous islands. These islands did not form sheets or membranes, nor. did they attach to either the posterior or the anterior retinal surface. The cortical fibrillar meshwork had reformed at 61 days' recovery; however, the condensed fibrillar material against the lens and filling the spaces between the ciliary processes had not resorbed. Neither shearing of the cortical gel or fibrillar matrix nor congestion of the anterior vitreous was observed in eyes only partially filled with gas. Springer-Verlag, 1990 Panessa-Warren Barbara Department of Allied Health Resources, Health Sciences Center, State University of New York, 11974, Stony Brook, NY, USA aut Maisel James Division of Ophthalmology, Nassau County Medical Center, 11544, East Meadow, Long Island, NY, USA aut Warren John Division of Instrumentation, Brookhaven National Laboratory, 11973, Upton, NY, USA aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 541-551 0721-832X 228:6<541 1990 228 417 https://doi.org/10.1007/BF00918488 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00918488 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Panessa-Warren Barbara Department of Allied Health Resources, Health Sciences Center, State University of New York, 11974, Stony Brook, NY, USA aut NATIONALLICENCE 700 1- Maisel James Division of Ophthalmology, Nassau County Medical Center, 11544, East Meadow, Long Island, NY, USA aut NATIONALLICENCE 700 1- Warren John Division of Instrumentation, Brookhaven National Laboratory, 11973, Upton, NY, USA aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 541-551 0721-832X 228:6<541 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer