caa a22 4500 465777457 CHVBK 20180323111946.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00918492 doi (NATIONALLICENCE)springer-10.1007/BF00918492 Culture of retinal pigment epithelial cells from subretinal fluid [Elektronische Daten] [Vappu Sirén, Eeva-Marjatta Salonen, Antti Vaheri, Petri Tommila, Ilkka Immonen] Culture of cells from subretinal fluid (SRF) was performed using 29 SRF samples obtained at retinal reattachment surgery. Proliferating cells were found in 58.6% of the samples studied. The cells were of retinal pigment epithelial (RPE) origin, as evidenced by their brown pigmentation in primary culture and their positive immunostaining for cytokeratins 8/18. The age of the patients did not affect the proliferative capacity of the cells. Proliferating cells were present in all samples from eyes with proliferative vitreoretinopathy (PVR) of grade C1 or more. In primary culture the cells had a fibroblast-like morphology, resembling that of ordinary RPE cells exposed to the vitreous. We conclude that the SRF of many patients with PVR contains viable proliferating RPE cells and that SRF offers a new source of RPE cells for studies on the pathogenesis of PVR. Springer-Verlag, 1990 Sirén Vappu Department of Virology, University of Helsinki, Haartmaninkatu 4, SF-00290, Helsinki, Finland aut Salonen Eeva-Marjatta Department of Virology, University of Helsinki, Haartmaninkatu 4, SF-00290, Helsinki, Finland aut Vaheri Antti Department of Virology, University of Helsinki, Haartmaninkatu 4, SF-00290, Helsinki, Finland aut Tommila Petri Department of Ophthalmology, University of Helsinki, Haartmaninkatu 4, SF-00290, Helsinki, Finland aut Immonen Ilkka Department of Ophthalmology, University of Helsinki, Haartmaninkatu 4, SF-00290, Helsinki, Finland aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 569-572 0721-832X 228:6<569 1990 228 417 https://doi.org/10.1007/BF00918492 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00918492 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sirén Vappu Department of Virology, University of Helsinki, Haartmaninkatu 4, SF-00290, Helsinki, Finland aut NATIONALLICENCE 700 1- Salonen Eeva-Marjatta Department of Virology, University of Helsinki, Haartmaninkatu 4, SF-00290, Helsinki, Finland aut NATIONALLICENCE 700 1- Vaheri Antti Department of Virology, University of Helsinki, Haartmaninkatu 4, SF-00290, Helsinki, Finland aut NATIONALLICENCE 700 1- Tommila Petri Department of Ophthalmology, University of Helsinki, Haartmaninkatu 4, SF-00290, Helsinki, Finland aut NATIONALLICENCE 700 1- Immonen Ilkka Department of Ophthalmology, University of Helsinki, Haartmaninkatu 4, SF-00290, Helsinki, Finland aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 569-572 0721-832X 228:6<569 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer