caa a22 4500 465777465 CHVBK 20180323111946.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00918483 doi (NATIONALLICENCE)springer-10.1007/BF00918483 Biochemical studies on human corneal proteoglycans —a comparison of normal and keratoconic eyes [Elektronische Daten] [Joseph Wollensak, Eckhart Buddecke] Human corneas from normal (healthy) donors and patients with keratoconus were either metabolically labelled under organ culture conditions or investigated without preincubation. The sulfated proteoglycans were isolated from a 4M guanidinium chloride/2% Triton X 100 extract. Two predominant proteoglycans were obtained from normal cornea after digestion of total sulfated proteoglycans with chondroitin ABC-lyase or endo-β-galactosidase. One had an overall mass of 150 kDa, two dermatan sulfate chains (M r≈50 kDa) with an iduronic acid content of 24%-28% and, after chondroitin ABC-lyase digestion, a core protein of 48 kDa. The other proteoglycan had an overall mass of 110 kDa, one keratan sulfate chain of ≈60 kDa and, following endo-β-galactosidase (keratanase) digestion, a core protein of 46 kDa. Each proteoglycan population was further fractionated into two subpopulations by chromatography on concanavalin A-Sepharose. The dermatan sulfateand keratan sulfate-containing proteoglycans isolated from keratoconic and healthy cornea had comparableM r values and core proteins with identical molecular weights, but the ratio of dermatan sulfate/keratan sulfate proteoglycan was increased in keratoconic cornea and the keratan sulfate chains of two keratan sulfate proteoglycans from keratoconic cornea were considerably shorter (M r 44 and 33 kDa) than those from normal corneas. Springer-Verlag, 1990 Wollensak Joseph Klinikum Charlottenburg, Augenklinik der Freien Universität, Spandauer Damm 130, 1000, Berlin 19, Germany aut Buddecke Eckhart Klinikum Charlottenburg, Augenklinik der Freien Universität, Spandauer Damm 130, 1000, Berlin 19, Germany aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 517-523 0721-832X 228:6<517 1990 228 417 https://doi.org/10.1007/BF00918483 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00918483 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wollensak Joseph Klinikum Charlottenburg, Augenklinik der Freien Universität, Spandauer Damm 130, 1000, Berlin 19, Germany aut NATIONALLICENCE 700 1- Buddecke Eckhart Klinikum Charlottenburg, Augenklinik der Freien Universität, Spandauer Damm 130, 1000, Berlin 19, Germany aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 517-523 0721-832X 228:6<517 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer