caa a22 4500 465777511 CHVBK 20180323111946.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00918490 doi (NATIONALLICENCE)springer-10.1007/BF00918490 Induction of MHC class II antigen in cultured bovine ciliary epithelial cells [Elektronische Daten] [Horst Helbig, Rebecca Gurley, Robert Reichl, Rashid Mahdi, Robert Nussenblatt, Alan Palestine] The expression of major histocompatibility complex (MHC) class II antigens in cultured bovine ciliary epithelial cells was investigated by means of indirect immunohistochemistry and immunocytofluorometry. Ciliary epithelial cells grown in control tissue-culture medium did not express MHC class II. However, after incubation with bovine gamma-interferon (IFN-G) in concentrations as low as 0.3 units/ml, nearly all cells stained for MHC class II. Tumor necrosis factor increased IFN-G-induced MHC class II expression. A reduction in IFN-G-induced MHC class II expression was observed with dexamethasone, prostaglandin EZ and alpha-interferon. To test whether MHC class II expression in response to IFN-G was specific for the ciliary epithelium, several intraocular tissues were grown in culture and incubated with IFN-G. MHC class II expression was observed in all tissues tested for response to IFN-G, but at different sensitivities. Retinal pigment epithelium and ciliary epithelium exhibited the highest sensitivity, followed by corneal endothelium and lens epithelium; the lowest sensitivity was observed for retinal vascular pericytes. The results are discussed in the context of MHC class II expression on the ciliary epithelium in anterior uveitis. Springer-Verlag, 1990 Helbig Horst Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut Gurley Rebecca Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut Reichl Robert Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut Mahdi Rashid Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut Nussenblatt Robert Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut Palestine Alan Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 556-561 0721-832X 228:6<556 1990 228 417 https://doi.org/10.1007/BF00918490 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00918490 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Helbig Horst Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut NATIONALLICENCE 700 1- Gurley Rebecca Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut NATIONALLICENCE 700 1- Reichl Robert Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut NATIONALLICENCE 700 1- Mahdi Rashid Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut NATIONALLICENCE 700 1- Nussenblatt Robert Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut NATIONALLICENCE 700 1- Palestine Alan Laboratory of Immunology, National Eye Institute, NIH, Bethesda, Md., USA aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 556-561 0721-832X 228:6<556 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer