caa a22 4500 465777538 CHVBK 20180323111946.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00918493 doi (NATIONALLICENCE)springer-10.1007/BF00918493 Effects of subconjunctival 5-fluorouracil injections on the corneal endothelium and ciliary epithelium [Elektronische Daten] [Makoto Araie, Yutaka Nakano, Takayuki Akahoshi] The effects of 14 successive daily subconjunctival 5-fluorouracil (5-FU) injections on corneal endothelial and ciliary epithelial cells were studied in pigmented rabbits. A total of 100 ltl 5% or 1.25% 5-FU was injected subconjunctivally in one eye and vehicle solution was injected in the other eye. At 12 h or 14 days after the 14th and last injection, both eyes were processed for observation with scanning or transmission electron microscopy. In other groups of rabbits injected with 5% 5-FU, effects on the corneal thickness, corneal endothelial permeability, blood-aqueous barrier permeability, intraocular pressure and aqueous flow rate were also studied. In the fellow eyes and treated eyes examined 14 days after the last injection, no significant morphological changes were seen. In eyes examined 12 h after the last injection, the following findings were obtained. The corneal endothelial cells showed moderate cytoplasmic swelling and mitochondrial swelling and vacuolation. The changes were dose-dependent and most marked in the area near the injection site, whereas they were minimal in the area apposing it. In the ciliary body adjacent to the injection site, disarrangement of the basal infoldings of non-pigmented epithelial cells was seen in eyes injected with 5% 5-FU, whereas no significant change was observed in those injected with 1.25% 5-FU. However, the physiological parameters examined remained unaltered. Springer-Verlag, 1990 Araie Makoto Department of Ophthalmology, University of Tokyo School of Medicine, 7-3-1, Hongo, 113, Bunkyo-ku, Tokyo, Japan aut Nakano Yutaka Department of Ophthalmology, University of Tokyo School of Medicine, 7-3-1, Hongo, 113, Bunkyo-ku, Tokyo, Japan aut Akahoshi Takayuki Department of Ophthalmology, University of Tokyo School of Medicine, 7-3-1, Hongo, 113, Bunkyo-ku, Tokyo, Japan aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 573-581 0721-832X 228:6<573 1990 228 417 https://doi.org/10.1007/BF00918493 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00918493 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Araie Makoto Department of Ophthalmology, University of Tokyo School of Medicine, 7-3-1, Hongo, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Nakano Yutaka Department of Ophthalmology, University of Tokyo School of Medicine, 7-3-1, Hongo, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Akahoshi Takayuki Department of Ophthalmology, University of Tokyo School of Medicine, 7-3-1, Hongo, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/6(1990-11-01), 573-581 0721-832X 228:6<573 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer