caa a22 4500 465777864 CHVBK 20180323111947.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00920064 doi (NATIONALLICENCE)springer-10.1007/BF00920064 Regenerative and proliferative capacity of adult human retinal cells in vitro [Elektronische Daten] [Solon Thanos, Hans-Jürgen Thiel] The present work was undertaken (a) to determine whether adult human retinal neurons possess the ability to survive and regrow neurites in organ cultures and (b) to investigate the maintenance and proliferative activity of retinal glial cells in vitro. Using material from retinae obtained from human eyes postenucleation we established in vitro organ cultures of retinal pieces in a chemically defined, serum-free medium, previously developed for culturing adult rat and chick retinae. The time course of glial cell migration and of neurite extension was compared with that of adult rat and chick retinae which have been extensively investigated in our laboratory. It appeared from four explanted retinae that the human retinal cells survive for up to 4 weeks in culture and exhibit their typical morphologies. Immunohistochemical investigation of the migrating cells revealed that both astrocytes and Müller-like cells exhibit their typical morphologies in vitro. In explants obtained from a retina 30 years after very traumatic violence to the eye cup, the glial cells but not the neurons extended lengthy fibers. In the explants obtained from a retina about 2 months after traumatic optic nerve injury and subsequent ocular complications, several lengthy fibres were extended from the transplant's edge. They could be labeled with neurofilament antibodies but not with glial fibrillary acidic protein antibodies, indicating their neuronal origin. The results suggest that some injured human retinal neurons respond to exposure to growthpermissive substrates by regeneration of their neurites. Although some morphological features of the fibers suggest that they originate from ganglion cells in the retinal explants, definitive proof of this is not yet available. Springer-Verlag, 1990 Thanos Solon Forschungslabor, Augenklinik, Universität Tübingen, Schleichstrasse 12, D-7400, Tübingen, Germany aut Thiel Hans-Jürgen Forschungslabor, Augenklinik, Universität Tübingen, Schleichstrasse 12, D-7400, Tübingen, Germany aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/4(1990-07-01), 369-376 0721-832X 228:4<369 1990 228 417 https://doi.org/10.1007/BF00920064 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00920064 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thanos Solon Forschungslabor, Augenklinik, Universität Tübingen, Schleichstrasse 12, D-7400, Tübingen, Germany aut NATIONALLICENCE 700 1- Thiel Hans-Jürgen Forschungslabor, Augenklinik, Universität Tübingen, Schleichstrasse 12, D-7400, Tübingen, Germany aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/4(1990-07-01), 369-376 0721-832X 228:4<369 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer