caa a22 4500 46577797X CHVBK 20180323111947.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00927264 doi (NATIONALLICENCE)springer-10.1007/BF00927264 Müller cells in detached human retina express glial fibrillary acidic protein and vimentin [Elektronische Daten] [Morio Okada, Miyo Matsumura, Nobuchika Ogino, Yoshihito Honda] We investigated the expression of vimentin and glial fibrillary acidic protein (GFAP) within Müller cells in normal human retinas and in detached human retinas of proliferative vitreoretinopathy (PVR) cases using the immunogold method. Muller cells in normal retinas showed vimentin immunoreactivity and faint GFAP immunoreactivity; however, in detached retinas they showed distinct GFAP immunoreactivity as well as vimentin immunoreactivity. Immunoelectron microscopic observation revealed that intermediate filaments (IF) within Müller cells in normal retinas showed vimentin immunoreactivity and that those within Müller cells in detached retinas showed both vimentin and GFAP immunoreactivity. Double staining for vimentin and GFAP showed that in detached retinas, these two protein immunoreactivities were observed in the same filaments. These results indicate that IF of human Müller cells consist of vimentin under normal conditions and that Müller cells in detached retinas contain different IF, which consist of vimentin and GFAP. Springer-Verlag, 1990 Okada Morio Department of Ophthalmology, Faculty of Medicine, Kyoto University, 606, Sakyo-ku, Kyoto, Japan aut Matsumura Miyo Department of Ophthalmology, Faculty of Medicine, Kyoto University, 606, Sakyo-ku, Kyoto, Japan aut Ogino Nobuchika Department of Ophthalmology, Aichi Medical University, 480-11, Aichi, Japan aut Honda Yoshihito Department of Ophthalmology, Faculty of Medicine, Kyoto University, 606, Sakyo-ku, Kyoto, Japan aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/5(1990-09-01), 467-474 0721-832X 228:5<467 1990 228 417 https://doi.org/10.1007/BF00927264 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00927264 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okada Morio Department of Ophthalmology, Faculty of Medicine, Kyoto University, 606, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Matsumura Miyo Department of Ophthalmology, Faculty of Medicine, Kyoto University, 606, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Ogino Nobuchika Department of Ophthalmology, Aichi Medical University, 480-11, Aichi, Japan aut NATIONALLICENCE 700 1- Honda Yoshihito Department of Ophthalmology, Faculty of Medicine, Kyoto University, 606, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/5(1990-09-01), 467-474 0721-832X 228:5<467 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer