caa a22 4500 465778194 CHVBK 20180323111948.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00935733 doi (NATIONALLICENCE)springer-10.1007/BF00935733 Retinal ischemia and cell proliferation in the rat: the role of soluble mitogens [Elektronische Daten] [Akihiro Ohira, Einar Stefansson, Eugene de Juan, Robert Davidson, Nicholas Faberowski] Temporary retinal ischemia in the rat leads to a proliferation of endothelial cells and glial cells. We tested the hypothesis that this proliferation is caused by a release of soluble mitogens from the ischemic retina. Conditioned media were prepared from normal rat retina and from retina that had undergone 2 h ischemia and 48 h reperfusion, at which time it showed intense mitotic activity. The conditioned media were placed in cultures of fibroblasts, bovine adrenal capillary endothelial cells, and rat brain astrocytes. Cell proliferation in vitro was stimulated by the retinal extracts in all cell cultures. However, the cell proliferation in cultures with conditioned media from normal retina was similar to that in cultures with conditioned media from ischemic and proliferating retina. Although these data are consistent with the presence of soluble growth factors in the retina, they also indicate that release of these growth factors into the surrounding milieu after transient retinal ischemia is not altered to a degree that would explain the dramatic increase in mitosis. Springer-Verlag, 1990 Ohira Akihiro Department of Ophthalmology, Duke University Medical Center, Box 3802, 27710, Durham, NC, USA aut Stefansson Einar University of Iceland, Reykjavik, Iceland aut de Juan Eugene Department of Ophthalmology, Duke University Medical Center, Box 3802, 27710, Durham, NC, USA aut Davidson Robert Department of Ophthalmology, Duke University Medical Center, Box 3802, 27710, Durham, NC, USA aut Faberowski Nicholas Department of Ophthalmology, Duke University Medical Center, Box 3802, 27710, Durham, NC, USA aut Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/2(1990-03-01), 195-199 0721-832X 228:2<195 1990 228 417 https://doi.org/10.1007/BF00935733 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00935733 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohira Akihiro Department of Ophthalmology, Duke University Medical Center, Box 3802, 27710, Durham, NC, USA aut NATIONALLICENCE 700 1- Stefansson Einar University of Iceland, Reykjavik, Iceland aut NATIONALLICENCE 700 1- de Juan Eugene Department of Ophthalmology, Duke University Medical Center, Box 3802, 27710, Durham, NC, USA aut NATIONALLICENCE 700 1- Davidson Robert Department of Ophthalmology, Duke University Medical Center, Box 3802, 27710, Durham, NC, USA aut NATIONALLICENCE 700 1- Faberowski Nicholas Department of Ophthalmology, Duke University Medical Center, Box 3802, 27710, Durham, NC, USA aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/2(1990-03-01), 195-199 0721-832X 228:2<195 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 465777139 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer