caa a22 4500 465778208 CHVBK 20180323111948.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00935731 doi (NATIONALLICENCE)springer-10.1007/BF00935731 Krauss Raimund Augenklinik und Institut für Pathologie und Physiologie der Rheinisch-Westfälischen Technischen Hochschule (RWTH), D-5100, Aachen, Germany aut New technique to demonstrate the network of blood capillaries of the human retina in their three-dimensional arrangement [Elektronische Daten] [Raimund Krauss] A method is presented that demonstrates the whole network of the blood capillaries of the human retina, making it possible (using a dark-field microscope at high magnification) to view the three-dimensional arrangement of this network in different planes by moving the drive of the microscope. The basic principle includes filling the vascular system with oxygen in statu nascendi by injection of H202 into the vitreous body of the intact eye and achieving fixation of the retinal tissue by application of a mixture of H202 and ethanol, and rendering the dry and flat retinal preparation transparent by soaking it in a specially prepared resin. The retinal vessels are made visible in an optically empty dark field by diffraction of light in a gas-filled specimen. This method allows preparation of the human retinal vasculature in simply enucleated eyes without cannulation of the central retinal artery and, therefore, it may be appropriate to support pathological and anatomical investigations. Springer-Verlag, 1990 Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/2(1990-03-01), 187-190 0721-832X 228:2<187 1990 228 417 https://doi.org/10.1007/BF00935731 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00935731 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Krauss Raimund Augenklinik und Institut für Pathologie und Physiologie der Rheinisch-Westfälischen Technischen Hochschule (RWTH), D-5100, Aachen, Germany aut NATIONALLICENCE 773 0- Graefe's Archive for Clinical and Experimental Ophthalmology Springer-Verlag 228/2(1990-03-01), 187-190 0721-832X 228:2<187 1990 228 417 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 465777112 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer