caa a22 4500 465781535 CHVBK 20180323111956.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF02918474 doi (NATIONALLICENCE)springer-10.1007/BF02918474 Structural requirements for the interaction between class II MHC molecules and peptide antigens [Elektronische Daten] [Alessandro Sette, Søren Buus, Ettore Appella, Luciano Adorini, Howard Grey] Summary: Previous work from our and other laboratories indicates that T cells recognize a complex between the MHC restriction element and peptide antigen fragments. This paper reviews the structural characteristics of the formation of such a complex. By analyzing in detail the interactions between purified IAd and IEd molecules and their peptide ligands, we found that some structural characteristics apply to both antigen-MHC interactions. In particular, we found: (1) each MHC molecule is capable of binding many unrelated peptides through the same peptide-binding site; (2) despite this permissiveness of binding, it is possible to define certain structural features of peptides that are associated with the capacity to bind to a particular MHC specificity (IAd or IEd); (3) IAd and IEd molecules recognize different and independent structures on the antigen molecule; (4) only ≈10% of the single amino acid substitutions tested on two IAd-and IEd-binding peptides had significant effect on their MHC-binding capacities, while over 80% of these substitutions significantly impaired T cell recognition of the Ia-peptide complex; (5) based on the segregation between residues that are crucial for T cell activation and Ia binding, the easiest model for the antigen-Ia-T-cell-receptor complex pictures the antigen molecule sandwiched in a planar conformation between the MHC and the T cell. Humana Press Inc., 1990 Sette Alessandro Cytel, 11099 North Torrey Pines Road, 92038, La Jolla, CA, USA aut Buus Søren University of Copenhagen, Copenhagen, Denmark aut Appella Ettore Laboratory of Cell Biology, National Cancer Institute, Bethesda, Md., USA aut Adorini Luciano Preclinical Research, Sandoz Ltd., Basel, Switzerland aut Grey Howard Cytel, 11099 North Torrey Pines Road, 92038, La Jolla, CA, USA aut Immunologic Research Humana Press 9/1(1990-03-01), 2-7 0257-277X 9:1<2 1990 9 12026 https://doi.org/10.1007/BF02918474 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02918474 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sette Alessandro Cytel, 11099 North Torrey Pines Road, 92038, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Buus Søren University of Copenhagen, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Appella Ettore Laboratory of Cell Biology, National Cancer Institute, Bethesda, Md., USA aut NATIONALLICENCE 700 1- Adorini Luciano Preclinical Research, Sandoz Ltd., Basel, Switzerland aut NATIONALLICENCE 700 1- Grey Howard Cytel, 11099 North Torrey Pines Road, 92038, La Jolla, CA, USA aut NATIONALLICENCE 773 0- Immunologic Research Humana Press 9/1(1990-03-01), 2-7 0257-277X 9:1<2 1990 9 12026 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer