caa a22 4500 465783376 CHVBK 20180323112001.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF02442676 doi (NATIONALLICENCE)springer-10.1007/BF02442676 Optimisation of enzyme electrodes [Elektronische Daten] [L. Tang, P. Vadgama] Enzyme electrodes have been fabricated in a variety of ways and used successfully for aqueous solution measurement. Knowledge about the fundamental basis of their operation has increased substantially in recent years, but the final, partly technological, hurdle of adaptation for practical use remains to be fully surmounted. Key aspects requiring attention are dependence on solution parameters capable of changing the kinetics of the immobilised enzyme reaction, direct fouling at the enzyme electrode, and the distorting effect of the biological matrix, notably blood and tissue, on the electrode environment due to the deposition of surface-active macromolecules and cellular elements. This review of enzyme electrode optimisation deals primarily with the various membranes which have been used to try to overcome such highly practical limitations to the biomedical use of enzyme electrodes. The classical amperometric glucose electrode based on detection of H2O2/O2 by the underlying sensor will be emphasised, but pH-based sensors for pyruvate and urea will also be discussed. The amperometric systems described indicate that with appropriate covering membranes, many, though by no means all, inferfacing problems can be overcome, and also that the type of optimisation engineered is dictated by the specific application envisaged. IFMBE, 1990 Biocompatibility nationallicence Cellulose acetate nationallicence Enzyme electrodes nationallicence Glucose electrode nationallicence Polycarbonate nationallicence Polydrethane nationallicence Pyruvate electrode nationallicence Tang L. Department of Medicine (Clinical Biochemistry), University of Manchester, Hope Hospital, Eccles Old Road, Salford, M6 8HD, Manchester, UK aut Vadgama P. Department of Medicine (Clinical Biochemistry), University of Manchester, Hope Hospital, Eccles Old Road, Salford, M6 8HD, Manchester, UK aut Medical and Biological Engineering and Computing Kluwer Academic Publishers 28/3(1990-05-01), B18-B24 0140-0118 28:3<B18 1990 28 11517 https://doi.org/10.1007/BF02442676 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02442676 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tang L. Department of Medicine (Clinical Biochemistry), University of Manchester, Hope Hospital, Eccles Old Road, Salford, M6 8HD, Manchester, UK aut NATIONALLICENCE 700 1- Vadgama P. Department of Medicine (Clinical Biochemistry), University of Manchester, Hope Hospital, Eccles Old Road, Salford, M6 8HD, Manchester, UK aut NATIONALLICENCE 773 0- Medical and Biological Engineering and Computing Kluwer Academic Publishers 28/3(1990-05-01), B18-B24 0140-0118 28:3<B18 1990 28 11517 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer