caa a22 4500 465783414 CHVBK 20180323112001.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF02442672 doi (NATIONALLICENCE)springer-10.1007/BF02442672 Blood plasma proteins on polyurethane and alkylsiloxane plasma-treated polyurethane surfaces. Dynamic approach by stimulus-response technique [Elektronische Daten] Part 2 evaluation of adsorption data by moment technique [M. Mutlu, E. Piskin] In this study, interactions of blood proteins (i.e. albumin and fibrinogen) with polyurethane biomaterial surfaces were investigated in an in vitro bead column test circuit using a stimulus-response technique. The dynamic sorption process of radiolabelled proteins on the surfaces was followed by detecting the radioactivity at the exit stream of the column, which was the response of a pulse stimulus at the inlet. The mathematical model was described and solved using ‘parameter estimation by cybernetic moment technique', and the adsorption rate constants of plasma proteins on different biomaterial surfaces were calculated. By evaluation of the response curves with standard and cybernetic moment techniques, the following results were obtained. Albumin and fibrinogen adsorption is competitive, and the competition is strongly dependent upon the surface characteristics of the biomaterial. Preadsorption or preferential adsorption of albumin decreases the fibrinogen adsorption, and there-fore increases the biocompatibility of material surface. Adsorption of blood plasma proteins are irreversible. The moment technique can also be used for the evaluation of stimulus-response data of biological systems, to determine the process parameters. IFMBE, 1990 Biomaterials nationallicence Blood plasma proteins nationallicence Moment technique nationallicence Polyurethane nationallicence Stimulus-response technique nationallicence Surface modification by plasma polymerisation nationallicence α 0 : a coefficient in eqn. 13 nationallicence C : tracer concentration, mol ml−1 nationallicence c av,i : average concentration of ith species, mol cm−3 nationallicence D : axial dispersion coefficient, cm2 s−1 nationallicence ∈ : void fraction nationallicence HMDS : hydroxymethyldisiloxane nationallicence HSA : human serum albumin nationallicence HSF : human serum fibrinogen nationallicence J : dimensionless concentration nationallicence k A : adsorption rate constant, cm3 g−1 s−1 nationallicence L : length of the packed bed, cm nationallicence M : amount of tracer injected nationallicence M 1 : first absolute moment,m 1/m 0,s nationallicence M 2 # : second central moment nationallicence m 0 : zeroth moment nationallicence m E 0 : experimental zeroth moment nationallicence m 1 E : experimental first moment, s nationallicence m 2 E : experimental second moment, s2 nationallicence m n : nth moment about the origin, eqn. 6 nationallicence N A ′ : net flux to surface, mol g−1 s−1 nationallicence Pe : Peclet number nationallicence PlzP(HMDS) : plasma polymerisation treated with HMDS nationallicence PSS : polished stainless-steel nationallicence PU : polyurethane nationallicence ρ p : particle density, g cm−3 nationallicence s : Laplace variable, s−1 nationallicence t : time,s nationallicence t 0 : injection time of pulse, s nationallicence U : interstitial velocity, cm s−1 nationallicence ζ : dimensionless length nationallicence z : axial length, cm nationallicence Mutlu M. Department of Chemical Engineering, Faculty of Engineering, Hacettepe University, 06532 Beytape Campus, Ankara, Turkey aut Piskin E. Department of Chemical Engineering, Faculty of Engineering, Hacettepe University, 06532 Beytape Campus, Ankara, Turkey aut Medical and Biological Engineering and Computing Kluwer Academic Publishers 28/3(1990-05-01), 232-236 0140-0118 28:3<232 1990 28 11517 https://doi.org/10.1007/BF02442672 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02442672 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mutlu M. Department of Chemical Engineering, Faculty of Engineering, Hacettepe University, 06532 Beytape Campus, Ankara, Turkey aut NATIONALLICENCE 700 1- Piskin E. Department of Chemical Engineering, Faculty of Engineering, Hacettepe University, 06532 Beytape Campus, Ankara, Turkey aut NATIONALLICENCE 773 0- Medical and Biological Engineering and Computing Kluwer Academic Publishers 28/3(1990-05-01), 232-236 0140-0118 28:3<232 1990 28 11517 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer