caa a22 4500 465784658 CHVBK 20180323112004.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00811436 doi (NATIONALLICENCE)springer-10.1007/BF00811436 Analytical and clinical evaluation of a new one-step non-analogue radioimmunoassay for serum-free thyroxine [Elektronische Daten] [Rémy Sapin, Françoise Gasser, Jean-Louis Schlienger, Jacques Chambron] We evaluated analytically and clinically the new one-step non-analogue free thyroxine (FT4) assay (Amerlex-MAB from Amersham), using a labelled monoclonal thyroxine-specific antibody as tracer, in comparison with the Gammacoat two-step FT4 kit (Baxter). Analytical performances of the new kit were excellent: within and between run coefficients of variation were < 5% in the working range. Clinical sensitivities for hypo- and hyperthyroidism were comparable for both kits (FT4 Amerlex-MAB 95% confidence interval: 12-25 pM). When serum was supplemented with albumin we observed a slight decrease in FT4 values measured by both kits. When oleate was added to serum we noted a moderate increase with the Amerlex-MAB kit up to 10 mM oleate added and a much more marked increase with the two-step kit. Results obtained with patients from particular euthyroid populations, known to have low albumin or high free fatty acids concentrations or to have perturbed FT4 results when measured by an analogue-based method, agreed with those of the in vitro studies. With these patients the specificity of the Amerlex-MAB FT4 results was good but slightly decreased compared with the two-step FT4 method, except for heparin-treated patients who were all classified according to their euthyroidal status (17/17 instead of 13/17 with the two-step kit). Springer-Verlag, 1990 Free thyroxine nationallicence Non-analogue radioimmunoassay nationallicence Effect of albumin nationallicence Effect of oleic acid nationallicence Thyroid status nationallicence Sapin Rémy Institut de Physique Biologique, Faculté de Médecine, 4 rue Kirschleger, F-67085, Strasbourg Cédex, France aut Gasser Françoise Institut de Physique Biologique, Faculté de Médecine, 4 rue Kirschleger, F-67085, Strasbourg Cédex, France aut Schlienger Jean-Louis Service de Médecine Interne, Hôpital de Flautepierre, F-67091, Strasbourg Cédex, France aut Chambron Jacques Institut de Physique Biologique, Faculté de Médecine, 4 rue Kirschleger, F-67085, Strasbourg Cédex, France aut European Journal of Nuclear Medicine Springer-Verlag 17/3-4(1990-03-01), 111-115 0340-6997 17:3-4<111 1990 17 259 https://doi.org/10.1007/BF00811436 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00811436 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sapin Rémy Institut de Physique Biologique, Faculté de Médecine, 4 rue Kirschleger, F-67085, Strasbourg Cédex, France aut NATIONALLICENCE 700 1- Gasser Françoise Institut de Physique Biologique, Faculté de Médecine, 4 rue Kirschleger, F-67085, Strasbourg Cédex, France aut NATIONALLICENCE 700 1- Schlienger Jean-Louis Service de Médecine Interne, Hôpital de Flautepierre, F-67091, Strasbourg Cédex, France aut NATIONALLICENCE 700 1- Chambron Jacques Institut de Physique Biologique, Faculté de Médecine, 4 rue Kirschleger, F-67085, Strasbourg Cédex, France aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 17/3-4(1990-03-01), 111-115 0340-6997 17:3-4<111 1990 17 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer