caa a22 4500 465784917 CHVBK 20180323112005.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00819401 doi (NATIONALLICENCE)springer-10.1007/BF00819401 Radiochemical purity of d,l hexamethylpropylene amine oxime and analysis of urine-excreted HMPAO decomposition species [Elektronische Daten] [Velimir Ivančević, Zeljko Kušter, Zora Maštrović, Darko Ivancevic] The radiochemical purity of hexamethylproxy-propylene amine oxime (HMPAO) was determined in 16 preparations using the three-strip method. Immediately post-formulation, 90.3%±4.0% (mean ±SD) of the activity was associated with the primary lipophilic complex having an Rf of 0.45 ± 0.11. We recorded a significantly higher content of sodium pertechnetate Tc 99m in methylethyl ketone (MEK) (21.1 % ± 8.5%) than in saline (5.0%+3.7%;P<0.001). To clarify this finding, we ran sequential chromatograms (t=0, 1, 2, 3 h) and found that the primary complex steadily disappeared, with a rate constant of 0.31 h−1. These results suggest that there is a decomposition of the primary complex during chromatography in MEK that might be responsible for the larger fraction of sodium pertechnetate Tc 99m in MEK. Eluate composition might influence the Rf of the lipophilic complex and the rate of its in vitro decomposition. In another experimental setting, we investigated99mTc-HMPAO decomposition species in urine after application of a suspension of labelled leukocytes by performing sequential chromatographic analyses in 11 patients. At I h after application, urinary activity reflected the presence of a secondary complex (84.8%±9.2%) and sodium pertechnetate Tc 99m (15.2% ±9.2%). The values after 3 h were markedly different (91.4%±4.8% and 8.6%±4.8%;P < 0.001). Thus, urinary activity mostly consisted of a secondary complex, increasing with time. Springer-Verlag, 1990 Radiochemical purity nationallicence hexamethylproxypropylene amine oxime nationallicence Urine chromatography nationallicence Ivančević Velimir Department of Nuclear Medicine, University Hospital Rebro, Kišpatićeva 12, YU-41000, Zagreb, Yugoslavia aut Kušter Zeljko Department of Nuclear Medicine, University Hospital Rebro, Kišpatićeva 12, YU-41000, Zagreb, Yugoslavia aut Maštrović Zora Department of Nuclear Medicine, University Hospital Rebro, Kišpatićeva 12, YU-41000, Zagreb, Yugoslavia aut Ivancevic Darko Department of Nuclear Medicine, University Hospital Rebro, Kišpatićeva 12, YU-41000, Zagreb, Yugoslavia aut European Journal of Nuclear Medicine Springer-Verlag 17/1-2(1990-01-01), 34-37 0340-6997 17:1-2<34 1990 17 259 https://doi.org/10.1007/BF00819401 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00819401 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ivančević Velimir Department of Nuclear Medicine, University Hospital Rebro, Kišpatićeva 12, YU-41000, Zagreb, Yugoslavia aut NATIONALLICENCE 700 1- Kušter Zeljko Department of Nuclear Medicine, University Hospital Rebro, Kišpatićeva 12, YU-41000, Zagreb, Yugoslavia aut NATIONALLICENCE 700 1- Maštrović Zora Department of Nuclear Medicine, University Hospital Rebro, Kišpatićeva 12, YU-41000, Zagreb, Yugoslavia aut NATIONALLICENCE 700 1- Ivancevic Darko Department of Nuclear Medicine, University Hospital Rebro, Kišpatićeva 12, YU-41000, Zagreb, Yugoslavia aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 17/1-2(1990-01-01), 34-37 0340-6997 17:1-2<34 1990 17 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer