caa a22 4500 465785018 CHVBK 20180323112006.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00819404 doi (NATIONALLICENCE)springer-10.1007/BF00819404 Kinetics of heat-damaged homologous erythrocytes [Elektronische Daten] A five-compartmental analysis [Panagiotis Dimitriou, Alfred Depascouale, Anastasios Germenis, Spiros-Emilios Antipas] A new theoretical five-compartmental model (5CM) was developed for analysis of the clearance of heat-damaged erythroctes (HDE) labelled with chromium 51. Besides the HDE-spleen interaction, this new model also takes into account the interaction between extrasplenic reticuloendothelial (RES) sites and HDE, i.e. the hepatic clearance of fragmented erythrocytes (FE). Accordingly, HIDE clearance curves are analysed into three exponential components, the fastest of which describes the RES-FE interaction, whereas the others describe the splenic clearance of spherocytes. Therefore, an estimation of the effective liver blood flow for HDE (ELBF) was achieved, along with a series of parameters describing splenic function. The 5CM proved to be more efficient than a previously proposed three-compartmental model (3CM) in the mathematical description of HDE clearance. Comparison was made by applying both models to 37 experimental curves obtained from 20 patients with congenital hemolytic anemias. The values for the splenic function parameters calculated by 5CM analysis and the strong correlations observed among them offer evidence that this model provides an adequate approximation to the real conditions under which HDE clearance takes place. Furthermore, a detailed quantitative analysis of the pooling of spherocytes within the spleen was attempted in this work, and this phenomenon was found to compete with splenic irreversible spherocyte trapping. The ELBF proved to be closely correlated with the hemodynamic splenic parameters, following first-order kinetics, as do low-dose colloids. Springer-Verlag, 1990 Heat-damaged erythrocyte kinetics nationallicence Spleen blood flow nationallicence Splenic pooling nationallicence Effective liver blood flow nationallicence Dimitriou Panagiotis Laboratory of Nuclear Medicine, Department of Pathologic Physiology, University of Athens, GR-115 27, Athens, Greece aut Depascouale Alfred Laboratory of Nuclear Medicine, Department of Pathologic Physiology, University of Athens, GR-115 27, Athens, Greece aut Germenis Anastasios Laboratory of Nuclear Medicine, Department of Pathologic Physiology, University of Athens, GR-115 27, Athens, Greece aut Antipas Spiros-Emilios Laboratory of Nuclear Medicine, Department of Pathologic Physiology, University of Athens, GR-115 27, Athens, Greece aut European Journal of Nuclear Medicine Springer-Verlag 17/1-2(1990-01-01), 49-54 0340-6997 17:1-2<49 1990 17 259 https://doi.org/10.1007/BF00819404 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00819404 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dimitriou Panagiotis Laboratory of Nuclear Medicine, Department of Pathologic Physiology, University of Athens, GR-115 27, Athens, Greece aut NATIONALLICENCE 700 1- Depascouale Alfred Laboratory of Nuclear Medicine, Department of Pathologic Physiology, University of Athens, GR-115 27, Athens, Greece aut NATIONALLICENCE 700 1- Germenis Anastasios Laboratory of Nuclear Medicine, Department of Pathologic Physiology, University of Athens, GR-115 27, Athens, Greece aut NATIONALLICENCE 700 1- Antipas Spiros-Emilios Laboratory of Nuclear Medicine, Department of Pathologic Physiology, University of Athens, GR-115 27, Athens, Greece aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 17/1-2(1990-01-01), 49-54 0340-6997 17:1-2<49 1990 17 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer