caa a22 4500 465785042 CHVBK 20180323112006.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00819402 doi (NATIONALLICENCE)springer-10.1007/BF00819402 Reduced technetium 99m labelled NCA-95/CEA-antibody uptake in liver due to gentle antibody reconstitution [Elektronische Daten] Technical note [S. Reske, U. Buell] The influence of reconstituting a murine monoclonal IgG1 antibody kit with pertechnetate technetium 99m on antibody distribution in the liver, spleen and sternal bone marrow of patients was examined. The99mTc-labelled antibody used is directed against nonspecific cross-reacting antigen (NCA-95) and carcinoembryonic antigen (CEA) and has been successfully applied for imaging tissue inflammation and bone marrow scanning. Radioactivity uptake was determined in the liver, spleen, bone marrow and a precordial background region in a consecutive series of 25 patients, examined with an antibody preparation, routinely radiolabelled according to the manufacturer's recommendations and in 14 patients, in whom the antibody was reconstituted with special care, avoiding bubble formation and dropping of buffer into the antibody-containing vial. Gentle compared with routine antibody reconstitution caused a highly significant reduction of the antibody uptake in the liver, as determined by count densities, normalized to injected dose and acquisition time (13.2 ± 5.5 vs 20.1 ± 6.0 cpm per pixel, x±SID,P=0.008). The liver to background ratio was reduced from 3.4 ± 1.4 to 1.9 ±0.5 (P < 0.001). Spleen, sternal bone marrow and precordial background count rates were not significantly affected. These results clearly demonstrate that gentle antibody reconstitution can decrease non-specific antibody uptake in the liver by 34% ± 6.4% (x ± SEM). Thus, scan quality is improved, and the potential deleterious camouflage of underlying structures is avoided. Springer-Verlag, 1990 Monoclonal antigranulocyte antibody nationallicence NCA-95-specific antibody nationallicence Bone marrow scintigraphy nationallicence Antibody liver uptake nationallicence Reske S. Department of Nuclear Medicine, RWTH Aachen, Pauwelsstrasse, D-5100, Aachen, Germany aut Buell U. Department of Nuclear Medicine, RWTH Aachen, Pauwelsstrasse, D-5100, Aachen, Germany aut European Journal of Nuclear Medicine Springer-Verlag 17/1-2(1990-01-01), 38-41 0340-6997 17:1-2<38 1990 17 259 https://doi.org/10.1007/BF00819402 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00819402 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reske S. Department of Nuclear Medicine, RWTH Aachen, Pauwelsstrasse, D-5100, Aachen, Germany aut NATIONALLICENCE 700 1- Buell U. Department of Nuclear Medicine, RWTH Aachen, Pauwelsstrasse, D-5100, Aachen, Germany aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 17/1-2(1990-01-01), 38-41 0340-6997 17:1-2<38 1990 17 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer