caa a22 4500 465785050 CHVBK 20180323112006.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF01268020 doi (NATIONALLICENCE)springer-10.1007/BF01268020 Effect of isoelectric point on biodistribution and inflammation imaging with indium-111-labelled IgG [Elektronische Daten] [Caroline Kate, Alan Fischman, Robert Rubin, A. Fucello, D. Riexinger, Robert Wilkinson, Lina Du, Ban Khaw, H. Strauss] Electrostatic effects play an important role in protein interactions and may alter the biodistribution of antibodies. To study the effect of molecular charge on the biodistribution and infection imaging properties of human polyclonal immunoglobulin G (IgG), its iso electric point was varied by changing the level of diethylene triamine penta-acetic acid (DTPA) substitution: 0.8, 0.9, 3.7, 5.1 and 5.9 DTPA/IgG. Biodistributions of the different IgG preparations were determined at 10 min, 1, 6, 24, and 48 h post injection in normal rats, and infection imaging properties were determined in rats withEscherichia coli thigh infections. The biodistribution was significantly affected by pl. The immunoglobulin preparations with 0.9 and 3.7 DTPA/IgG showed faster clearance from the circulation and generally lower accumulation in most organs. The images had a target-to-background ratio of approximately 1.3-2.3:1. These results suggest that even though targeting is not affected by the level of DTPA substitutions, preparations with 0.9 and 3.7 DTPA/IgG may be superior imaging agents because of reduced accumulation by background organs. Springer-Verlag, 1990 Indium nationallicence IgG nationallicence Diethylene triamine penta-acetic acid nationallicence Infection nationallicence Isoelectric point nationallicence Radiolabelledantibody nationallicence Biodistribution nationallicence Kate Caroline Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut Fischman Alan Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut Rubin Robert Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut Fucello A. Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut Riexinger D. Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut Wilkinson Robert Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut Du Lina Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut Khaw Ban Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut Strauss H. Division of Nuclear Medicine, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut European Journal of Nuclear Medicine Springer-Verlag 17/6-8(1990-06-01), 305-309 0340-6997 17:6-8<305 1990 17 259 https://doi.org/10.1007/BF01268020 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01268020 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kate Caroline Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut NATIONALLICENCE 700 1- Fischman Alan Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut NATIONALLICENCE 700 1- Rubin Robert Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut NATIONALLICENCE 700 1- Fucello A. Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut NATIONALLICENCE 700 1- Riexinger D. Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut NATIONALLICENCE 700 1- Wilkinson Robert Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut NATIONALLICENCE 700 1- Du Lina Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut NATIONALLICENCE 700 1- Khaw Ban Department of Radiology and Infectious Disease Unit of the Medical Service, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut NATIONALLICENCE 700 1- Strauss H. Division of Nuclear Medicine, Massachusetts General Hospital 32, Fruit Street, Massachusetts, USA aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 17/6-8(1990-06-01), 305-309 0340-6997 17:6-8<305 1990 17 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer