caa a22 4500 465785107 CHVBK 20180323112006.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF01268018 doi (NATIONALLICENCE)springer-10.1007/BF01268018 Inhibition of the hepatocyte uptake of radiolabelled monoclonal antibodies by chelating agents [Elektronische Daten] [B. Davidson, P. Boulos, J. Porter] The imaging of small abdominal tumours with indium 111 labelled monoclonal antibodies (MAbs) is often obscured by the uptake of activity into the hepto cytes of normal liver tissue. A model has therefore been developed to analyse reagents which may inhibit the he patocyte uptake of111In-MAb whilst preserving tumour uptake. Primary rat hepatocyte cultures and an epithelial membrane antigen (EMA) expressing tumour cell line (MCF7), recognised by the EMA-specific MAb ICR2, were obtained in tissue culture. Monolayers of both cells were incubated with the111In-MAb with or without the additional reagents and the cell uptake then measured and expressed per milligram of cell protein using a Lowry protein assay. No preferential reduction in hepatocyte uptake was noted by incubating cells with either saturated or unsaturated transferrin. The chelating agent, diethylene triamine penta-acetic acid (DTPA), however, significantly reduced the uptake of activity in hepatocytes but not the tumour cell line (TP<0.05). An optimum concentration and time period for incubating DTPA with labelled MAb was established. The mean hepatocyte uptake was reduced by 80% with a 1 h incubation with 1 mM DTPA. These results suggest that DTPA may have a role in reducing the liver uptake of radioactivity in patient studies using111In-MAb. Springer-Verlag, 1990 Immunoscintigraphy nationallicence Indium 111 nationallicence Monoclonal antibody nationallicence Chelating agents nationallicence Davidson B. Department of Surgery, University College and Middlesex School of Medicine, London, UK aut Boulos P. Department of Surgery, University College and Middlesex School of Medicine, London, UK aut Porter J. Department of Haematology, University College and Middlesex School of Medicine, London, UK aut European Journal of Nuclear Medicine Springer-Verlag 17/6-8(1990-06-01), 294-298 0340-6997 17:6-8<294 1990 17 259 https://doi.org/10.1007/BF01268018 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01268018 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Davidson B. Department of Surgery, University College and Middlesex School of Medicine, London, UK aut NATIONALLICENCE 700 1- Boulos P. Department of Surgery, University College and Middlesex School of Medicine, London, UK aut NATIONALLICENCE 700 1- Porter J. Department of Haematology, University College and Middlesex School of Medicine, London, UK aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 17/6-8(1990-06-01), 294-298 0340-6997 17:6-8<294 1990 17 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer