caa a22 4500 465785387 CHVBK 20180323112007.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00812360 doi (NATIONALLICENCE)springer-10.1007/BF00812360 Mouse-specific antibody responses to a monoclonal antibody during repeated immunoscintigraphy investigations: Comparison of antibody titres and imaging studies in a rat model [Elektronische Daten] [Malcolm Pimm, Alan Perkins, Sandra Gribben, Amanda Markham] As a model for human mouse-specific antibody responses in patients undergoing immunoscintigraphy, we have investigated in rats the production of mouse-specific antibodies (MA) to the mouse monoclonal antibody 791T/36. At intervals of between 5 and 16 weeks the rats were given repeated cycles of intravenous (IV) injections of antibody with or without a simultaneous intradermal (ID) injection. The IV dose was 60 gmg/kg, a dose similar to that used in many clinical immunoscintigraphy studies. The ID injection was 2 μg, which mimicks the skin test dose often given in clinical imaging protocols. The study was carried out with both131I-labelled antibody and with antibody labelled with111In by diethylenetriamine-penta-acetic acid (DTPA) chelation. MA was measured with a passive haemagglutination assay using sheep red blood cells coated with the monoclonal antibody. Of rats given ID injections of unlabelled antibody at the same time as the IV imaging doses, 9/20 produced MA during 4 cycles of injections. In contrast, only 2/16 rats given only the IV dose produced MA. Both131I- and111In-labelled antibody appeared equally immunogenic with 5/18 and 6/18 overall responders, respectively. The production of MA was associated with a significant perturbation in the biodistribution of the IV dose of labelled antibody as seen by gamma-camera imaging of the rats given111In-labelled antibody. There was clearance of immune complexes to the liver, this organ accumulating up to 90% of the whole body count rate of radiolabel. MA titres of between 1/100 and 1/78000 caused equal perturbation of biodistribution, although below 1/100 the effect was more variable. Springer-Verlag, 1990 Monoclonal antibody nationallicence Immunoscintigraphy nationallicence Mouse-specific antibody nationallicence Pimm Malcolm Cancer Research Campaign Laboratories, University of Nottingham, Nottingham, UK aut Perkins Alan Department of Medical Physics, University Hospital, Nottingham, UK aut Gribben Sandra Cancer Research Campaign Laboratories, University of Nottingham, Nottingham, UK aut Markham Amanda Cancer Research Campaign Laboratories, University of Nottingham, Nottingham, UK aut European Journal of Nuclear Medicine Springer-Verlag 17/5(1990-05-01), 216-222 0340-6997 17:5<216 1990 17 259 https://doi.org/10.1007/BF00812360 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00812360 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pimm Malcolm Cancer Research Campaign Laboratories, University of Nottingham, Nottingham, UK aut NATIONALLICENCE 700 1- Perkins Alan Department of Medical Physics, University Hospital, Nottingham, UK aut NATIONALLICENCE 700 1- Gribben Sandra Cancer Research Campaign Laboratories, University of Nottingham, Nottingham, UK aut NATIONALLICENCE 700 1- Markham Amanda Cancer Research Campaign Laboratories, University of Nottingham, Nottingham, UK aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 17/5(1990-05-01), 216-222 0340-6997 17:5<216 1990 17 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer