caa a22 4500 465785867 CHVBK 20180323112008.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00842783 doi (NATIONALLICENCE)springer-10.1007/BF00842783 In vitro comparison of HMPAO and gentisic acid for labelling leukocytes with99mTc [Elektronische Daten] [Mary Ecclestone, Annette Proulx, James Ballinger, Bonny Gerson, Robert Reid, Karen Gulenchyn] Leukocytes can be labelled with99mTc using HMPAO and gentisic acid methods. We compared the two methods with respect to labelling efficiency on mixed leukocytes and isolated polymorphonuclear (PMN) and mononuclear (MN) cells, and the in vitro stability of the label. HMPAO produced approximately 70% labelling efficiency on mixed or PMN cells and the label was stable in saline or plasma. Labelling efficiency on MN was only 14% and was less stable. Gentisic acid produced a labelling efficiency of 52% on PMN and 35% on MN, both were stable in saline but less stable in plasma. In conclusion, HMPAO produces higher labelling efficiency and the label shows greater in vitro stability in plasma. However, gentisic acid is much less expensive to use, allows labelling of MN cells, and should result in more favourable microdosimetry. Preliminary clinical results suggest that gentisic acid is equivalent to HMPAO but has the advantage of being much cheaper. Springer-Verlag, 1990 99mTc-HMPAO nationallicence Gentisic acid nationallicence Leukocyte labelling nationallicence Ecclestone Mary Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut Proulx Annette Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut Ballinger James Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut Gerson Bonny Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut Reid Robert Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut Gulenchyn Karen Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut European Journal of Nuclear Medicine Springer-Verlag 16/4-6(1990-04-01), 299-302 0340-6997 16:4-6<299 1990 16 259 https://doi.org/10.1007/BF00842783 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00842783 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ecclestone Mary Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Proulx Annette Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Ballinger James Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Gerson Bonny Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Reid Robert Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Gulenchyn Karen Division of Nuclear Medicine, Ottawa Civic Hospital, 1053 Carling Avenue, K1Y 4E9, Ottawa, Ontario, Canada aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 16/4-6(1990-04-01), 299-302 0340-6997 16:4-6<299 1990 16 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer