caa a22 4500 465786073 CHVBK 20180323112009.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00998161 doi (NATIONALLICENCE)springer-10.1007/BF00998161 123I-MIBG pulmonary removal: a biochemical marker of minimal lung endothelial cell lesions [Elektronische Daten] [Daniel Slosman, Barbara Polla, Alfred Donath] Circulating biogenic amines, such as serotonin or norepinephrine, are cleared by the lung and can be considered as indicators of pulmonary endothelial integrity. An iodinated norepinephrine analogue, metaiodo-benzylguanidine (MIBG), is extracted in the lung by an active, saturable transport system. In order to investigate the use of MIBG lung extraction to detect minimal,lung endothelial cell lesions, an experimental model of initial pulmonary vascular lesions was developed in rats using repeated daily intraperitoneal injection of bleomycin (BLM: 2 U/100 g body weight) over a 5-day period. At this time, a significant decrease of serum angiotensin-converting enzyme activity, an index of endothelial cell metabolism, was observed (214±11 U/ml in controls as compared with 182±11 U/ml in BLM-treated rats,P < 0.05); electron microscopy showed the presence of typical but minimal endothelial cell lesions (blebbing). MIBG extraction (%EMIBG) was measured using the isolated perfused lung model after a 10-min steady-state period and 2 min of perfusion with123I-MIBG (0.1 μCi/ml) and125I-labelled human serum albumin (HSA) (0.2 μCi/ml). Intraperitoneal administration of BLM to rats for 5 days resulted in a significant decrease in pulmonary extraction of MIBG. %EMIBG declined from a control value of 24.7%±1.1% in controls to 19.3%±1.6% in BLM-treated rats (n=27,P<0.05; -21.2%). HSA lung extraction, used as an estimate of nonspecific residual lung activity, was not different in the lungs of BLM-treated rats as compared with controls. Because measurements of MIBG lung extraction (%EMIBG) were able to demonstrate the presence of minimal endothelial cell lesions due to the BLM toxicity, MIBG is an index of pulmonary endothelial cell function. Springer-Verlag, 1990 Metaiodobenzylguanizide nationallicence Bleomycin lung toxicity nationallicence Lung metabolism nationallicence Slosman Daniel Division of Nuclear Medicine, Hôpital Cantonal Unviersitaire de Genève, CH-1211, Geneva 4, Switzerland aut Polla Barbara Respiratory Division, Hôpital Cantonal Unviersitaire de Genève, CH-1211, Geneva 4, Switzerland aut Donath Alfred Division of Nuclear Medicine, Hôpital Cantonal Unviersitaire de Genève, CH-1211, Geneva 4, Switzerland aut European Journal of Nuclear Medicine Springer-Verlag 16/8-10(1990-08-01), 633-637 0340-6997 16:8-10<633 1990 16 259 https://doi.org/10.1007/BF00998161 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00998161 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Slosman Daniel Division of Nuclear Medicine, Hôpital Cantonal Unviersitaire de Genève, CH-1211, Geneva 4, Switzerland aut NATIONALLICENCE 700 1- Polla Barbara Respiratory Division, Hôpital Cantonal Unviersitaire de Genève, CH-1211, Geneva 4, Switzerland aut NATIONALLICENCE 700 1- Donath Alfred Division of Nuclear Medicine, Hôpital Cantonal Unviersitaire de Genève, CH-1211, Geneva 4, Switzerland aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 16/8-10(1990-08-01), 633-637 0340-6997 16:8-10<633 1990 16 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer