caa a22 4500 465786286 CHVBK 20180323112010.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00998163 doi (NATIONALLICENCE)springer-10.1007/BF00998163 Investigation by scintigraphic methods of neutrophil kinetics under normal and septic shock conditions in the experimental baboon model [Elektronische Daten] [I. Dormehl, M. Maree, D. Cromarty, H. Böckmann, L. Jacobs, E. van Rensburg, J. Kilian] The purpose of this study was the correlation of neutrophil kinetics with the pathogenic course of septic shock in the baboon model. Radioactively labelled neutrophils were traced in vivo in normal baboons (n= 6) and inEscherichia coli-infected baboons, which were reinjected with labelled autologous neutrophils either 2 h after the onset of theE. coli infusion (procedure A) (n= 3) or simultaneously with the infusion (procedure B) (n=3). Cell isolation was done according to a method developed in this laboratory. The cells were labelled with tropolonate In 111. resuspended in 1-2 ml plasma and reinjected. One-minute images were taken every 5th min and then hourly for 4 h with a gamma camera and analysed with a data processor. Time-activity curves were obtained for neutrophil washout from the lungs and neutrophil accumulation in the liver and spleen. These curves were compared for normal baboons and for those treated according to procedures A and B. A significant retention of neutrophils in the lungs of baboons withE. coli-induced septic shock was noted as well as an abnormally slow rate of accumulation in the liver and spleen. It also seems that any lung injury which could be attributed to changes in neutrophil behaviour should be traced back to events during the early exposure of neutrophils to bacterial infection. Springer-Verlag, 1990 Labelled neutrophil kinetics nationallicence Septic shock nationallicence Baboon model nationallicence Dormehl I. AEC Institute for Life Sciences, Medical Faculty, University of Pretoria, South Africa aut Maree M. AEC Institute for Life Sciences, Medical Faculty, University of Pretoria, South Africa aut Cromarty D. AEC Institute for Life Sciences, Medical Faculty, University of Pretoria, South Africa aut Böckmann H. H A Grové Research Centre, Pretoria aut Jacobs L. AEC Institute for Life Sciences, Medical Faculty, University of Pretoria, South Africa aut van Rensburg E. AEC Institute for Life Sciences, Medical Faculty, University of Pretoria, South Africa aut Kilian J. Department of Internal Medicine, Medical Faculty, University of Pretoria, PO Box 2034, 0001, Pretoria, South Africa aut European Journal of Nuclear Medicine Springer-Verlag 16/8-10(1990-08-01), 643-647 0340-6997 16:8-10<643 1990 16 259 https://doi.org/10.1007/BF00998163 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00998163 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dormehl I. AEC Institute for Life Sciences, Medical Faculty, University of Pretoria, South Africa aut NATIONALLICENCE 700 1- Maree M. AEC Institute for Life Sciences, Medical Faculty, University of Pretoria, South Africa aut NATIONALLICENCE 700 1- Cromarty D. AEC Institute for Life Sciences, Medical Faculty, University of Pretoria, South Africa aut NATIONALLICENCE 700 1- Böckmann H. H A Grové Research Centre, Pretoria aut NATIONALLICENCE 700 1- Jacobs L. AEC Institute for Life Sciences, Medical Faculty, University of Pretoria, South Africa aut NATIONALLICENCE 700 1- van Rensburg E. AEC Institute for Life Sciences, Medical Faculty, University of Pretoria, South Africa aut NATIONALLICENCE 700 1- Kilian J. Department of Internal Medicine, Medical Faculty, University of Pretoria, PO Box 2034, 0001, Pretoria, South Africa aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 16/8-10(1990-08-01), 643-647 0340-6997 16:8-10<643 1990 16 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer