caa a22 4500 465786456 CHVBK 20180323112010.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF01465912 doi (NATIONALLICENCE)springer-10.1007/BF01465912 In vivo imaging of rat lymphocytes with an indium 111-labelled anti-T cell monoclonal antibody: a comparison with indium 111-labelled lymphocytes [Elektronische Daten] [Issa Loutfi, Patricia Chisholm, Debbie Bevan, John Lavender] An indium 111-labelled mouse anti-rat T cell monoclonal antibody, MRC OX-19, was injected intravenously into rats to establish the usefulness of radiolabelled anti-lymphocyte antibodies in imaging lymphoid tissues. Antibody binding in vivo, measured by immunofluorescence analysis of cell suspensions made from lymphoid tissues, was detectable on lymphocytes in blood, spleen and lymph nodes. The extent of binding was time and antibody-dose dependent. Doses of antibody above 80 μg/kg body weight resulted in modulation, i.e. loss of CD 5 (T 1) molecules from the cell surface, although the cells remained in the circulation. Modulation was demonstrable within 2 h and for at least 24 h after a single injection of antibody. Intravenous injection of111In-MRC OX-19 resulted in levels of in vivo binding comparable with those seen with unlabelled antibody. Scintillation imaging showed early splenic localisation persisting over 48h, a more gradual localisation in the lymph nodes seen clearly at 24 h and a steady background. Comparison of the in vivo distribution of labelled antibody and111In-tropolone-labelled lymphocytes showed that both could be used for external imaging of lymphocytes by scintillation camera. Springer-Verlag, 1990 Monoclonal antibody nationallicence T lymphocytes nationallicence CD 5 nationallicence 111In nationallicence scintillation camera imaging nationallicence Loutfi Issa Department of Nuclear Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 ONN, London, UK aut Chisholm Patricia Department of Nuclear Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 ONN, London, UK aut Bevan Debbie The Immunology Section, Division of Biomolecular Science, Kings College, University of London, London, UK aut Lavender John Department of Nuclear Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 ONN, London, UK aut European Journal of Nuclear Medicine Springer-Verlag 16/2(1990-02-01), 69-76 0340-6997 16:2<69 1990 16 259 https://doi.org/10.1007/BF01465912 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01465912 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Loutfi Issa Department of Nuclear Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 700 1- Chisholm Patricia Department of Nuclear Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 700 1- Bevan Debbie The Immunology Section, Division of Biomolecular Science, Kings College, University of London, London, UK aut NATIONALLICENCE 700 1- Lavender John Department of Nuclear Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 ONN, London, UK aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 16/2(1990-02-01), 69-76 0340-6997 16:2<69 1990 16 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer