caa a22 4500 465786588 CHVBK 20180323112011.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00833012 doi (NATIONALLICENCE)springer-10.1007/BF00833012 Detection of experimental thrombi in rabbits with an131I-labelled fibrin-specific monoclonal antibody [Elektronische Daten] [Karen Walker, Laurie Milner, George Bautovich, Peter Borham, Andrew Wood, Dennis Rylatt, Patricia Martin, Peter Bundesen, Graeme Boniface] The detection of thrombi in rabbits has been investigated with131I-labelled DD-3136/22, a monoclonal antibody (Mab) reactive at high affinity (Kd=2.68 x 10−10 M) with human D Dimer (DD). DD-3136/22 bound well to both "fresh” and "aged” human clots in an in vitro assay but showed poor binding to rabbit clots. However, reactivity was restored to rabbit blood if it was seeded, before clotting, with human DD covalently coupled to Sepharose beads. Thus, a rabbit model was developed in which blood was allowed to clot around DD-Sepharose beads introduced into the jugular vein. Gamma camera imaging showed that intact131I-labelled DD-3136/22 localised to these clots within 24 h. Uptake at this time was 0.202±0.012% injected dose per gram (%ID/g) compared with 0.086±0.018%ID/g after injection of control antibody.131I-labelled F(ab′)2, fragments of DD-3136/22 allowed earlier scintigraphic detection of the clot which was evident 4 h after injection. Uptake in the clot at 24 h was 0.154±0.038 %ID/g compared with 0.109±0.027 %ID/g for a control F(ab')2. As antigen levels in the clot are estimated to be less than 300 μg DD, thus representing a very small human clot, the DD-3136/22 Mab would appear to have a good potential for the sensitive detection of thrombi in a clinical setting. Springer-Verlag, 1990 Scintigraphy nationallicence Monoclonal antibody nationallicence Gamma camera imaging nationallicence Clots nationallicence Fibrin nationallicence Rabbit model nationallicence Walker Karen Clinical Immunology Research Centre, University of Sydney, 2006, Sydney, NSW, Australia aut Milner Laurie Clinical Immunology Research Centre, University of Sydney, 2006, Sydney, NSW, Australia aut Bautovich George Department of Nuclear Medicine, Royal Prince Alfred Hospital, NSW, Australia aut Borham Peter Department of Veterinary Clinical Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut Wood Andrew Department of Veterinary Clinical Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut Rylatt Dennis Agen Biomedical Ltd, Acacia Ridge, OLD, Australia aut Martin Patricia Agen Biomedical Ltd, Acacia Ridge, OLD, Australia aut Bundesen Peter Agen Biomedical Ltd, Acacia Ridge, OLD, Australia aut Boniface Graeme Australian Nuclear Science and Technology Organisation, Menai, NSW, Australia aut European Journal of Nuclear Medicine Springer-Verlag 16/11(1990-11-01), 787-794 0340-6997 16:11<787 1990 16 259 https://doi.org/10.1007/BF00833012 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00833012 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Walker Karen Clinical Immunology Research Centre, University of Sydney, 2006, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Milner Laurie Clinical Immunology Research Centre, University of Sydney, 2006, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Bautovich George Department of Nuclear Medicine, Royal Prince Alfred Hospital, NSW, Australia aut NATIONALLICENCE 700 1- Borham Peter Department of Veterinary Clinical Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Wood Andrew Department of Veterinary Clinical Sciences, University of Sydney, 2006, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Rylatt Dennis Agen Biomedical Ltd, Acacia Ridge, OLD, Australia aut NATIONALLICENCE 700 1- Martin Patricia Agen Biomedical Ltd, Acacia Ridge, OLD, Australia aut NATIONALLICENCE 700 1- Bundesen Peter Agen Biomedical Ltd, Acacia Ridge, OLD, Australia aut NATIONALLICENCE 700 1- Boniface Graeme Australian Nuclear Science and Technology Organisation, Menai, NSW, Australia aut NATIONALLICENCE 773 0- European Journal of Nuclear Medicine Springer-Verlag 16/11(1990-11-01), 787-794 0340-6997 16:11<787 1990 16 259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer