caa a22 4500 465787533 CHVBK 20180323112013.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00297325 doi (NATIONALLICENCE)springer-10.1007/BF00297325 Enzyme electrode based on gold-plated polyester cloth [Elektronische Daten] 2. NAD+ and NADH regeneration by diaphorase-immobilized electrode [Shigeru Kunugi, Kazuhito Ikeda, Takaya Nakashima, Hideyuki Yamada] Summary: Diaphorase is immobilized on electrodes derived from metal-plated polyester cloth by entrapment method and coupled with an electron mediator. Cyclic voltammogram depended on the existence of the substrate NADH (for oxidation) or NAD+ (for reduction) and the flavo-enzyme was shown to exchange electron(s) with the cloth electrode by mediation of the ferrocene derivative (for oxidation cycle) or the viologen derivative (for reduction cycle), even in the immobilized state. Coupling of the NADH or NAD+ regenerating system with alcohol dehydrogenase was examined. Springer-Verlag, 1990 Kunugi Shigeru Research Group of Enzyme Engineering, Department of Applied Chemistry and Biotechnology, Fukui University, 910, Fukui, Japan aut Ikeda Kazuhito Research Group of Enzyme Engineering, Department of Applied Chemistry and Biotechnology, Fukui University, 910, Fukui, Japan aut Nakashima Takaya Research Group of Enzyme Engineering, Department of Applied Chemistry and Biotechnology, Fukui University, 910, Fukui, Japan aut Yamada Hideyuki Seiren Co. Ltd., Keya, 910, Fukui, Japan aut Polymer Bulletin Springer-Verlag 24/2(1990-08-01), 247-250 0170-0839 24:2<247 1990 24 289 https://doi.org/10.1007/BF00297325 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00297325 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kunugi Shigeru Research Group of Enzyme Engineering, Department of Applied Chemistry and Biotechnology, Fukui University, 910, Fukui, Japan aut NATIONALLICENCE 700 1- Ikeda Kazuhito Research Group of Enzyme Engineering, Department of Applied Chemistry and Biotechnology, Fukui University, 910, Fukui, Japan aut NATIONALLICENCE 700 1- Nakashima Takaya Research Group of Enzyme Engineering, Department of Applied Chemistry and Biotechnology, Fukui University, 910, Fukui, Japan aut NATIONALLICENCE 700 1- Yamada Hideyuki Seiren Co. Ltd., Keya, 910, Fukui, Japan aut NATIONALLICENCE 773 0- Polymer Bulletin Springer-Verlag 24/2(1990-08-01), 247-250 0170-0839 24:2<247 1990 24 289 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer