caa a22 4500 465789420 CHVBK 20180323112019.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00216920 doi (NATIONALLICENCE)springer-10.1007/BF00216920 Discrepancy between cytotoxicity and DNA interstrand crosslinking of carboplatin and cisplatin in vivo [Elektronische Daten] [Wilfried DeNeve, Frederick Valeriote, Efstathios Tapazoglou, Carleen Everett, Anup Khatana, Thomas Corbett] We used the method of alkaline elution to compare quantitatively the DNA lesions produced by cisplatin and carboplatin in the AKR leukemia in vivo. These data were compared with cytotoxicity of each drug in the same animal model and in a solid tumor murine model (colon 26). DNA-protein and DNA-DNA interstrand crosslinks were formed in similar proportions by both drugs when peak values of crosslinking were compared. No clear difference in the rate of formation of both types of crosslinks could be observed between these drugs. On a molar basis a 3- to 4-fold more carboplatin had to be given to obtain equivalent frequencies of both types of crosslinks. In contrast, to obtain equitoxicity in the same animal tumor model, 13 fold higher doses of carboplatin had to be given. This difference in cytotoxicity between both drugs is comparable to the difference measured in colon 26 in vivo (16 fold). Both values are in the range of literature data (10-25 fold) dealing with the relative potency of cisplatin and carboplatin in murine tumor models. Kluwer Academic Publishers, 1990 in vivo DNA crosslinking nationallicence cisplatin nationallicence carboplatin nationallicence DIC : DNA-DNA Interstrand Crosslinks nationallicence DPC : Protein-DNA Crosslinks nationallicence EDTA : Ethylene-Diammine-Tetra-Acetic Acid nationallicence PK : Proteinase K nationallicence PSA : Puck's Saline A nationallicence SSB : Single Strand Breaks nationallicence ILS : Increase in Life Span nationallicence Carboplatin-Cis-(diammino)(1,1-cyclobutane-dicarboxylato)-Platinum (II), Cisplatin : Cis-Diamminedichloroplatinum (II) nationallicence DeNeve Wilfried Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut Valeriote Frederick Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut Tapazoglou Efstathios Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut Everett Carleen Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut Khatana Anup Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut Corbett Thomas Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut Investigational New Drugs Kluwer Academic Publishers 8/1(1990-02-01), 17-24 0167-6997 8:1<17 1990 8 10637 https://doi.org/10.1007/BF00216920 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00216920 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- DeNeve Wilfried Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut NATIONALLICENCE 700 1- Valeriote Frederick Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut NATIONALLICENCE 700 1- Tapazoglou Efstathios Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut NATIONALLICENCE 700 1- Everett Carleen Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut NATIONALLICENCE 700 1- Khatana Anup Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut NATIONALLICENCE 700 1- Corbett Thomas Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University School of Medicine, 48201, Detroit, MI, USA aut NATIONALLICENCE 773 0- Investigational New Drugs Kluwer Academic Publishers 8/1(1990-02-01), 17-24 0167-6997 8:1<17 1990 8 10637 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer