caa a22 4500 465789579 CHVBK 20180323112019.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00171835 doi (NATIONALLICENCE)springer-10.1007/BF00171835 Combination of flavone acetic acid (FAA) with adriamycin, cis-platinum and difluoromethylornithine (DFMO) in vitro against human colon cancer cells [Elektronische Daten] [Sarabjit Neelam, Alvise Bernabei, Curtis Freedland, Roxanna Thompson, Thomas Corbett, Gordon Luk] Summary: Unresectable solid tumors in the metastatic stage are quite resistant to current chemotherapy and radiation therapy regimens. Flavone acetic acid (FAA) is a novel antitumor agent which appears to work through a different mechanism than the conventional chemotherapeutic agents. In preclinical studies it has shown effectiveness against a variety of transplantable murine and human tumors and appears to be solid tumor selective. It also has non-overlapping toxicities as compared to conventional agents. We therefore investigated FAA in vitro against human colon cancer cells and explored whether its effectiveness could be enhanced in combination with other agents such as adriamycin (ADR), cis-platinum (CP) and difluoromethyornithine (DFMO) — an inhibitor of polyamine biosynthesis. Addition of FAA for 24 hours in liquid media produced dose dependent growth inhibition. Using soft agar colony assay, growth was inhibited by 58% by 3mM FAA and only 1.4% by 0.375mM FAA. The combination of FAA and cis-platinum produced synergism at the lower doses tested. The combination of FAA and adriamycin produced antagonism at all doses tested and the combination of FAA with DFMO did not produce results significantly different from DFMO alone. We conclude that enhancement of FAA activity can be achieved in combination with conventional antitumor agents, but may be drug and dose specific. Kluwer Academic Publishers, 1990 flavone acetic acid nationallicence adriamycin nationallicence cis-platinum nationallicence difluoromethylornithine nationallicence drug studies nationallicence Neelam Sarabjit Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut Bernabei Alvise Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut Freedland Curtis Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut Thompson Roxanna Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut Corbett Thomas Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut Luk Gordon Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut Investigational New Drugs Kluwer Academic Publishers 8/3(1990-08-01), 263-268 0167-6997 8:3<263 1990 8 10637 https://doi.org/10.1007/BF00171835 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00171835 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Neelam Sarabjit Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut NATIONALLICENCE 700 1- Bernabei Alvise Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut NATIONALLICENCE 700 1- Freedland Curtis Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut NATIONALLICENCE 700 1- Thompson Roxanna Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut NATIONALLICENCE 700 1- Corbett Thomas Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut NATIONALLICENCE 700 1- Luk Gordon Department of Medicine, Wayne State University School of Medicine and Harper Hospital Detroit, 48201, MI, USA aut NATIONALLICENCE 773 0- Investigational New Drugs Kluwer Academic Publishers 8/3(1990-08-01), 263-268 0167-6997 8:3<263 1990 8 10637 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer