caa a22 4500 465789749 CHVBK 20180323112020.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00171979 doi (NATIONALLICENCE)springer-10.1007/BF00171979 Schedule dependent potentiation of antitumor drug effects by α-difluoromethylornithine in human gastric carcinoma cells in vitro [Elektronische Daten] [Sam Barranco, Courtney Townsend Jr., Barbara Ho, Karen Reumont, Steven Koester, Pamella Ford] Summary: A clone of human gastric cancer cells (AGS-6) and the parental line (AGS-P) from which it was isolated were used in cell survival studies to determine whether pretreatment for 24, 48 or 72h with α-difluoromethylornithine (DFMO, 5mM) would increase the cell's sensitivity to 5-Fluorouracil (5FU), Adriamycin (Adria), 1-(2-chloroethyl)-3-(4-methyl cyclohexyl)-1-nitrosourea (MeCCNU), or Bleomycin (Bleo). Generally, the AGS parental cells were most sensitive to the anticancer agents after exposures to DFMO. However, there was no way to predict in advance from DFMO-induced changes in ornithine decarboxylase (ODC), polyamine or cell kinetics values, how long an exposure to DFMO was required before sensitization to an anticancer agent occurred. The degree of potentiation for a single drug was variable from time to time during exposure to DFMO, and broad differences in the sensitizations were demonstrated among the four anticancer drugs. The AGS-6 clone exhibited little or no increased sensitivity as a result of pretreatment with DFMO, even though the DFMO-induced reductions in ODC and polyamine values in these cells were similar to those produced in the more sensitive parental line. Kluwer Academic Publishers, 1990 stomach cancer nationallicence polyamines nationallicence αDFMO nationallicence heterogeneity nationallicence cell killing nationallicence Barranco Sam Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut Townsend Jr. Courtney Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut Ho Barbara Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut Reumont Karen Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut Koester Steven Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut Ford Pamella Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut Investigational New Drugs Kluwer Academic Publishers 8(1990-03-01), S9-S18 0167-6997 8<S9 1990 8 10637 https://doi.org/10.1007/BF00171979 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00171979 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barranco Sam Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut NATIONALLICENCE 700 1- Townsend Jr Courtney Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut NATIONALLICENCE 700 1- Ho Barbara Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut NATIONALLICENCE 700 1- Reumont Karen Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut NATIONALLICENCE 700 1- Koester Steven Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut NATIONALLICENCE 700 1- Ford Pamella Department of Radiation Oncology & Biophysics, Eastern Virginia Medical School, P.O. Box 1980, 23501-1980, Norfolk, VA, USA aut NATIONALLICENCE 773 0- Investigational New Drugs Kluwer Academic Publishers 8(1990-03-01), S9-S18 0167-6997 8<S9 1990 8 10637 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer