caa a22 4500 465790240 CHVBK 20180323112022.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF02015052 doi (NATIONALLICENCE)springer-10.1007/BF02015052 Oren Aharon The Division of Microbial and Molecular Ecology, The Institute of Life Sciences, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel aut Thymidine incorporation in saltern ponds of different salinities: Estimation of in situ growth rates of halophilic archaeobacteria and eubacteria [Elektronische Daten] [Aharon Oren] Incorporation of [methyl-3H]thymidine was measured in solar saltern ponds of different salinities. Estimated doubling times of the bacterial communities were in the range of 1.1 to 22.6 days. Even at the highest salt concentrations (NaCl saturation), relatively rapid thymidine incorporation was observed. In an attempt to differentiate between activity of halophilic archaeobacteria (theHalobacterium group) and halophilic eubacteria, taurocholate, which causes lysis of the halobacteria without affecting eubacteria, was used. At salt concentrations exceeding 250 g/liter all thymidine incorporation activity could be attributed to halobacteria. Aphidicolin, a potent inhibitor of DNA synthesis in halobacteria, completely abolished thymidine incorporation at the highest salinities, but also caused significant inhibition at salinities at which halobacteria are expected to be absent. Attempts to use nalidixic acid to selectively inhibit DNA synthesis by the eubacterial communities were unsuccessful. Springer-Verlag New York Inc., 1990 Microbial Ecology Springer-Verlag 19/1(1990-01-01), 43-51 0095-3628 19:1<43 1990 19 248 https://doi.org/10.1007/BF02015052 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02015052 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Oren Aharon The Division of Microbial and Molecular Ecology, The Institute of Life Sciences, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel aut NATIONALLICENCE 773 0- Microbial Ecology Springer-Verlag 19/1(1990-01-01), 43-51 0095-3628 19:1<43 1990 19 248 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer