caa a22 4500 465790305 CHVBK 20180323112022.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF02017171 doi (NATIONALLICENCE)springer-10.1007/BF02017171 Differential adhesion, activity, and carbohydrate: Protein ratios of Pseudomonas atlantica monocultures attaching to stainless steel in a linear shear gradient [Elektronische Daten] [M. Mittelman, D. Nivens, C. Low, D. White] Biofilm formation on metallic surfaces in marine and freshwater environments often precedes corrosion and other biofouling conditions. Attachment is mediated by such environmental factors as the presence of surface conditioning films, fluid dynamics, bulk-phase nutrient levels, and surface chemistry. In this study, we utilized a Fowler Cell Adhesion Measurement Module to demonstrate that the changes in cellular concentration and composition of a monoculture ofPseudomonas atlantica biofilms on stainless steel were a function of the applied shear force. At shear forces in the range of 3-10 dynes cm−2 (1.0 liter min−1), attachment as measured by acridine orange direct microscopic counts was greatest at the higher shear forces.14C-Acetate uptake activity on the stainless steel surfaces increased with shear stress. Acetate incorporation ranged from 1×10−5 to 19×10−5 μmol cm−2 between 0.15 and 30 dynes cm−2 for 30 min uptake periods. On a per cell basis, however, activity decreased with shear, indicating a shift in metabolism. Fourier transform infrared spectroscopy revealed that protein and carbohydrate concentrations also increased with the applied shear. Increased biofilm C∶N ratios and total fatty acids were associated with the higher shear stresses. Neither radius of interaction nor biofilm age appeared to significantly influence the relationship between fluid shear and attachment and cellular composition ofP. atlantica biofilms in the range of 1-10 dynes cm−2. Springer-Verlag New York Inc., 1990 Mittelman M. Institute for Applied Microbiology, University of Tennessee, 37923, Knoxville, Tennessee, USA aut Nivens D. Institute for Applied Microbiology, University of Tennessee, 37923, Knoxville, Tennessee, USA aut Low C. Institute for Applied Microbiology, University of Tennessee, 37923, Knoxville, Tennessee, USA aut White D. Institute for Applied Microbiology, University of Tennessee, 37923, Knoxville, Tennessee, USA aut Microbial Ecology Springer-Verlag 19/3(1990-05-01), 269-278 0095-3628 19:3<269 1990 19 248 https://doi.org/10.1007/BF02017171 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02017171 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mittelman M. Institute for Applied Microbiology, University of Tennessee, 37923, Knoxville, Tennessee, USA aut NATIONALLICENCE 700 1- Nivens D. Institute for Applied Microbiology, University of Tennessee, 37923, Knoxville, Tennessee, USA aut NATIONALLICENCE 700 1- Low C. Institute for Applied Microbiology, University of Tennessee, 37923, Knoxville, Tennessee, USA aut NATIONALLICENCE 700 1- White D. Institute for Applied Microbiology, University of Tennessee, 37923, Knoxville, Tennessee, USA aut NATIONALLICENCE 773 0- Microbial Ecology Springer-Verlag 19/3(1990-05-01), 269-278 0095-3628 19:3<269 1990 19 248 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer