caa a22 4500 465790313 CHVBK 20180323112022.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF02017168 doi (NATIONALLICENCE)springer-10.1007/BF02017168 Long-term survival of and plasmid stability in Pseudomonas and Klebsiella species and appearance of nonculturable cells in agricultural drainage water [Elektronische Daten] [L. van Overbeek, J. van Elsas, J. Trevors, M. Starodub] One year after introduction into agricultural drainage waterPseudomonas fluorescens R2f (RP4),Pseudomonas putida CYM318 (pRK2501), andKlebsiella aerogenes NCTC418 (pBR322) could be recovered on agar media. Survival of the introduced strains depended on competition with the indigenous microflora, the presence of nutrients, and the availability of air. In contrast toK. aerogenes NCTC418 (pBR322), bothPseudomonas species lost their plasmids, as indicated by the consistently lower colony counts on selective medium compared with the counts on nonselective medium. The plasmid loss did not depend on nutrient status and oxygen supply. P. fluorescens R2f cells could be detected with the immunofluorescence (IF) technique. Total cell counts determined by IF were consistently higher than corresponding colony counts. Even in samples where no colonies were recovered, R2f cells could be detected by IF. This indicated the occurrence of nonculturable R2f cells in drainage water. Homology with32P-labelled plasmid RP4 DNA was found in several drainage water samples that originally receivedP. fluorescens R2f (RP4), by using the cell suspension filter hybridization technique. P. putida CYM318 andK. aerogenes NCTC418 cells could also be detected in sterile drainage water samples, after nonspecific staining with fluorescein isothiocyanate. Cell counts of both strains were consistently higher than corresponding plate counts. Springer-Verlag New York Inc., 1990 van Overbeek L. Research Institute Ital, P.O. Box 48, 6700, AA Wageningen, The Netherlands aut van Elsas J. Research Institute Ital, P.O. Box 48, 6700, AA Wageningen, The Netherlands aut Trevors J. Department of Environmental Biology, University of Guelph, Chemistry-Microbiology Building, N1G 2W1, Guelph, Ontario, Canada aut Starodub M. Department of Environmental Biology, University of Guelph, Chemistry-Microbiology Building, N1G 2W1, Guelph, Ontario, Canada aut Microbial Ecology Springer-Verlag 19/3(1990-05-01), 239-249 0095-3628 19:3<239 1990 19 248 https://doi.org/10.1007/BF02017168 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02017168 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Overbeek L. Research Institute Ital, P.O. Box 48, 6700, AA Wageningen, The Netherlands aut NATIONALLICENCE 700 1- van Elsas J. Research Institute Ital, P.O. Box 48, 6700, AA Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Trevors J. Department of Environmental Biology, University of Guelph, Chemistry-Microbiology Building, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Starodub M. Department of Environmental Biology, University of Guelph, Chemistry-Microbiology Building, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 773 0- Microbial Ecology Springer-Verlag 19/3(1990-05-01), 239-249 0095-3628 19:3<239 1990 19 248 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer