caa a22 4500 465790380 CHVBK 20180323112022.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF02017170 doi (NATIONALLICENCE)springer-10.1007/BF02017170 Natural transformation of a marine Vibrio species by plasmid DNA [Elektronische Daten] [Wade Jeffrey, John Paul, Gregory Stewart] Vibrio sp. DI9, recently isolated from Tampa Bay, FL, has been found to be naturally transformed by the broad host range plasmid pKT230 in both filter transformation assays and sterile sediment microcosms. This is the first report of natural transformation by plasmid DNA of aVibrio sp. and of a marine bacterial isolate. Transformation frequencies ranged from 0.3 to 3.1×10−8 transformants per recipient. Transformants were detected by both plating and by selection for growth in liquid medium in the presence of streptomycin and kanamycin and confirmed by probing of southern transfers. Transformation was enhanced by multimeric forms of the plasmid. A technique using sediment microcosms, mixed populations ofVibrio sp. DI9 and another antibiotic resistant organism, and enrichment in liquid media has been developed which allows detection of transformation at frequencies too low to be detected by plating. This technique may serve as a model for the detection of natural transformation in the environment. These results suggest that natural transformation may be one mechanism of horizontal plasmid transfer in the marine environment, and may provide the methodology with which to detect this process in natural populations of bacteria. Springer-Verlag New York Inc., 1990 Jeffrey Wade Department of Marine Science, University of South Florida, 140 7th Ave. South, 33701, St. Petersburg, Florida aut Paul John Department of Biology, University of South Florida, 33620, Tampa, Florida aut Stewart Gregory Department of Biology, University of South Florida, 33620, Tampa, Florida aut Microbial Ecology Springer-Verlag 19/3(1990-05-01), 259-268 0095-3628 19:3<259 1990 19 248 https://doi.org/10.1007/BF02017170 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02017170 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jeffrey Wade Department of Marine Science, University of South Florida, 140 7th Ave. South, 33701, St. Petersburg, Florida aut NATIONALLICENCE 700 1- Paul John Department of Biology, University of South Florida, 33620, Tampa, Florida aut NATIONALLICENCE 700 1- Stewart Gregory Department of Biology, University of South Florida, 33620, Tampa, Florida aut NATIONALLICENCE 773 0- Microbial Ecology Springer-Verlag 19/3(1990-05-01), 259-268 0095-3628 19:3<259 1990 19 248 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer