caa a22 4500 465790402 CHVBK 20180323112022.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF02012095 doi (NATIONALLICENCE)springer-10.1007/BF02012095 A sensitive chromatographic method for the detection of pyruvyl groups in microbial polymers from sediments [Elektronische Daten] [James Smith, Ernesto Quintero, Gill Geesey] A method was developed for the quantitation of pyruvyl groups in microbial polymers using mild acid hydrolysis, o-phenylenediamine labeling, reversed-phase high-performance liquid chromatography (RP-HPLC), and fluorescence detection. The method was used to determine the pyruvate content of various microbial exopolysaccharides and to estimate the abundance of polymeric pyruvate in freshwater sediments. The results of this method were compared with those of several other pyruvate assays. The detection limit of the method was 1.6 nmol pyruvate. As little as 3.7μg of the bacterial polysaccharide xanthan gum, or from 5 to 22 mg of sediment (depending on polymeric pyruvate content), were needed for detection and quantitation of polymeric pyruvate. The results should be useful in determining the contribution of polymeric pyruvate to total metal-binding ligands in sediments. Springer-Verlag New York Inc., 1990 Smith James Department of Microbiology, California State University, Long Beach, 90840, Long Beach, California, USA aut Quintero Ernesto Department of Microbiology, California State University, Long Beach, 90840, Long Beach, California, USA aut Geesey Gill Department of Microbiology, California State University, Long Beach, 90840, Long Beach, California, USA aut Microbial Ecology Springer-Verlag 19/2(1990-03-01), 137-147 0095-3628 19:2<137 1990 19 248 https://doi.org/10.1007/BF02012095 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02012095 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smith James Department of Microbiology, California State University, Long Beach, 90840, Long Beach, California, USA aut NATIONALLICENCE 700 1- Quintero Ernesto Department of Microbiology, California State University, Long Beach, 90840, Long Beach, California, USA aut NATIONALLICENCE 700 1- Geesey Gill Department of Microbiology, California State University, Long Beach, 90840, Long Beach, California, USA aut NATIONALLICENCE 773 0- Microbial Ecology Springer-Verlag 19/2(1990-03-01), 137-147 0095-3628 19:2<137 1990 19 248 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer