caa a22 4500 465790429 CHVBK 20180323112022.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF02012094 doi (NATIONALLICENCE)springer-10.1007/BF02012094 High performance liquid chromatography detection of phototrophic bacterial pigments in aquatic environments [Elektronische Daten] [Y. Yacobi, Werner Eckert, H. Trüper, T. Berman] Pigment extracts of phototrophic bacteria isolated from Lake Kinneret (Rhodopseudomonas palustris, Thiocapsa roseopersicina, Prosthecochloris aestuaris andChlorobium phaeobacteroides) were studied by means of high performance liquid chromatography (HPLC). An absorption wavelength of 360 nm provided the best resolution among the pigments of the species tested and between them and chlorophylla. Signature pigments were identified for each of these species, and their presence was thereby monitored in lake water samples.C. phaeobacteroides, which was observed in the anaerobic hypolimnion and predominated in the metalimnion, was recognized by a characteristic cluster of major chlorophyllous pigment peaks. The spectral qualities of these pigments were close but not identical to published data on bacteriochlorophylle, presumably due to the use of different solvents for extraction. The intensity of these pigment peaks was employed to determine the depth of the greatest phototrophic bacterial biomass, which was not related to that of algae. Springer-Verlag New York Inc., 1990 Yacobi Y. Kinneret Limnological Laboratory, P.O. Box 345, 14102, Tiberias, Israel aut Eckert Werner Kinneret Limnological Laboratory, P.O. Box 345, 14102, Tiberias, Israel aut Trüper H. Institute of Microbiology, University of Bonn, Meckenheimer Allee 168, D-5300, Bonn-1, Federal Republic of Germany aut Berman T. Kinneret Limnological Laboratory, P.O. Box 345, 14102, Tiberias, Israel aut Microbial Ecology Springer-Verlag 19/2(1990-03-01), 127-136 0095-3628 19:2<127 1990 19 248 https://doi.org/10.1007/BF02012094 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02012094 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yacobi Y. Kinneret Limnological Laboratory, P.O. Box 345, 14102, Tiberias, Israel aut NATIONALLICENCE 700 1- Eckert Werner Kinneret Limnological Laboratory, P.O. Box 345, 14102, Tiberias, Israel aut NATIONALLICENCE 700 1- Trüper H. Institute of Microbiology, University of Bonn, Meckenheimer Allee 168, D-5300, Bonn-1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Berman T. Kinneret Limnological Laboratory, P.O. Box 345, 14102, Tiberias, Israel aut NATIONALLICENCE 773 0- Microbial Ecology Springer-Verlag 19/2(1990-03-01), 127-136 0095-3628 19:2<127 1990 19 248 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer