caa a22 4500 465790437 CHVBK 20180323112022.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF02012096 doi (NATIONALLICENCE)springer-10.1007/BF02012096 Habitable pore space and survival of Rhizobium leguminosarum biovar trifolii introduced into soil [Elektronische Daten] [J. Postma, J. van Veen] The hypothesis that the population size of introduced bacteria is affected by habitable pore space was studied by varying moisture content and bulk density in sterilized, as well as in natural loamy sand and silt loam. The soils were inoculated withRhizobium leguminosarum biovartrifolii and established and maintained at soil water potentials between −5 and −20 kPa (pF 1.7 and 2.3). Rhizobial cells were enumerated when population sizes were expected to be more or less stable. In sterilized soils, the rhizobial numbers were not affected or decreased only slightly when water potentials increased from −20 to −5 kPa. In natural soils, the decrease in rhizobial numbers with increasing water potentials was more pronounced. Bulk density had only minor effects on the population sizes of rhizobia or total bacteria. Soil water retention curves of both soils were used to calculate volume and surface area of pores from different diameter classes, and an estimation of the habitable pore space was made. Combining these values of the theoretical habitable pore space with the measured rhizobial numbers showed that only 0.37 and 0.44% of the habitable pore space was occupied in the sterilized loamy sand and silt loam, respectively. The situation in natural soil is more complicated, since a whole variety of microorganisms is present. Nevertheless, it was suggested that, in general, pore space does not limit proliferation and growth of soil microorganisms. Springer-Verlag New York Inc., 1990 Postma J. Research Institute Ital, Wageningen, The Netherlands aut van Veen J. Research Institute Ital, Wageningen, The Netherlands aut Microbial Ecology Springer-Verlag 19/2(1990-03-01), 149-161 0095-3628 19:2<149 1990 19 248 https://doi.org/10.1007/BF02012096 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02012096 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Postma J. Research Institute Ital, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- van Veen J. Research Institute Ital, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Microbial Ecology Springer-Verlag 19/2(1990-03-01), 149-161 0095-3628 19:2<149 1990 19 248 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer