caa a22 4500 465790453 CHVBK 20180323112022.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF02012098 doi (NATIONALLICENCE)springer-10.1007/BF02012098 Dynamic interactions of Pseudomonas aeruginosa and bacteriophages in lake water [Elektronische Daten] [Oladele Ogunseitan, Gary Sayler, Robert Miller] The persistence and interaction between newly isolated strains ofPseudomonas aeruginosa and resident bacteriophages indigenous to a freshwater environment was monitored over 45 days in lake water microcosms. The interaction between susceptible and resistant bacteria with pure phage (UT1) particles or a mixed phage population (M1) was investigated by following temporal changes in host density, phage-to-bacteria ratio (PBR), and the appearance of apparent prophage carriers within the host population. Decay rates of the phage (UT1) ranged from 0.054 hour−1 in natural water to 0.027 hour−1 in filtered lake water. About 45% of sensitive bacteria incubated with phase UT1 were pseudolysogenic within 12 hours of incubation in natural lake water. This process was delayed until 72 hours in the steile lake water control, suggesting that host-phage interaction is promoted in the presence of a viable natural microbial community. Phage UT1 appeared to stabilize the density of host bacteria in lake water at a level of 104 colony-forming units (cfu) ml−1. Bacterial coexistence with the mixed phage (M1) population resulted in an oscillating equilibrium with the PBR stabilizing at about 3. The presence of extraneous homoimmune phages appeared to be detrimental to the stability of the pseudolysogens, which were maintained at a lower population density than prophage-free cells in lake water containing the mixed phage (M1) population. Springer-Verlag New York Inc., 1990 Ogunseitan Oladele Department of Microbiology and The Graduate Program in Ecology, Center for Environmental Biotechnology, University of Tennessee, 37932-2567, Knoxville, Tennessee aut Sayler Gary Department of Microbiology and The Graduate Program in Ecology, Center for Environmental Biotechnology, University of Tennessee, 37932-2567, Knoxville, Tennessee aut Miller Robert Department of Biochemistry and Biophysics, The Program in Molecular Biology, Stritch School of Medicine, Loyola University of Chicago, 60153, Maywood, Illinois, USA aut Microbial Ecology Springer-Verlag 19/2(1990-03-01), 171-185 0095-3628 19:2<171 1990 19 248 https://doi.org/10.1007/BF02012098 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02012098 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ogunseitan Oladele Department of Microbiology and The Graduate Program in Ecology, Center for Environmental Biotechnology, University of Tennessee, 37932-2567, Knoxville, Tennessee aut NATIONALLICENCE 700 1- Sayler Gary Department of Microbiology and The Graduate Program in Ecology, Center for Environmental Biotechnology, University of Tennessee, 37932-2567, Knoxville, Tennessee aut NATIONALLICENCE 700 1- Miller Robert Department of Biochemistry and Biophysics, The Program in Molecular Biology, Stritch School of Medicine, Loyola University of Chicago, 60153, Maywood, Illinois, USA aut NATIONALLICENCE 773 0- Microbial Ecology Springer-Verlag 19/2(1990-03-01), 171-185 0095-3628 19:2<171 1990 19 248 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer