caa a22 4500 465790534 CHVBK 20180323112023.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02543864 doi (NATIONALLICENCE)springer-10.1007/BF02543864 Correlation of nonspecific macromolecular labeling with environmental parameters during [3H]Thymidine incorporation in the waters of southwest florida [Elektronische Daten] [Wade Jeffrey, John Paul, Lisa Cazares, Mary DeFlaun, Andrew David] During routine [3H]thymidine incorporation measurements of environmental samples, significant amounts of radioactivity are often incorporated into macromolecules other than DNA. Although the percentage of nonspecific labeling varies both temporally and spatially, the cause(s) of these variations remain unknown. Correlations between the percent incorporated radioactivity in DNA and a variety of experimental and environmental parameters measured in the Alfia River, Crystal River, Medard Reservoir, and Bayboro Harbor were examined. The amount of radioactivity incorporated into DNA ranged from 6 to 95% ( $$\bar x = 51 \pm 14\% $$ ; n=121). Nonspecific labeling began immediately upon the addition of [3H]thymidine and was linear over time. Labeling patterns were independent of both the amount of thymidine added and cell-size fraction. A two year study of Bayboro Harbor indicated no conclusive relationship between nonspecific labeling and seasonality. The amount of radioactivity incorporated into DNA was inversely correlated with total rates of thymidine incorporation and a strong diurnal pattern was observed in the Crystal River. No consistent relationship was observed between labeling patterns and primary productivity, chlorophylla, particulate DNA, dissolved DNA, bacterial cell numbers, temperature, salinity, and dissolved organic carbon. The only relationship with dissolved inorganic nutrients (N and P) occurred in the Crystal River. In this phosphate limited river, the percent of radioactivity incorporated into DNA was positively correlated with phosphate concentrations. These results indicate that nonspecific labeling is not dependent on any one parameter but may be a function of many interacting environmental factors or a function of the specific ambient bacterial population. Springer-Verlag New York Inc., 1989 Jeffrey Wade Department of Marine Science, University of South Florida, 33701, St. Petersburg, Florida, USA aut Paul John Department of Marine Science, University of South Florida, 33701, St. Petersburg, Florida, USA aut Cazares Lisa Department of Marine Science, University of South Florida, 33701, St. Petersburg, Florida, USA aut DeFlaun Mary Department of Marine Science, University of South Florida, 33701, St. Petersburg, Florida, USA aut David Andrew Department of Marine Science, University of South Florida, 33701, St. Petersburg, Florida, USA aut Microbial Ecology Springer-Verlag 20/1(1990-12-01), 21-35 0095-3628 20:1<21 1990 20 248 https://doi.org/10.1007/BF02543864 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02543864 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jeffrey Wade Department of Marine Science, University of South Florida, 33701, St. Petersburg, Florida, USA aut NATIONALLICENCE 700 1- Paul John Department of Marine Science, University of South Florida, 33701, St. Petersburg, Florida, USA aut NATIONALLICENCE 700 1- Cazares Lisa Department of Marine Science, University of South Florida, 33701, St. Petersburg, Florida, USA aut NATIONALLICENCE 700 1- DeFlaun Mary Department of Marine Science, University of South Florida, 33701, St. Petersburg, Florida, USA aut NATIONALLICENCE 700 1- David Andrew Department of Marine Science, University of South Florida, 33701, St. Petersburg, Florida, USA aut NATIONALLICENCE 773 0- Microbial Ecology Springer-Verlag 20/1(1990-12-01), 21-35 0095-3628 20:1<21 1990 20 248 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer