caa a22 4500 465790585 CHVBK 20180323112023.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02543875 doi (NATIONALLICENCE)springer-10.1007/BF02543875 Transformation of indole by methanogenic and sulfate-reducing microorganisms isolated from digested sludge [Elektronische Daten] [Rishi Shanker, Jean-Marc Bollag] In the present study, mineralization of an aromaticN-heterocyclic molecule, indole, by microorganisms present in anaerobically digested sewage sludge was examined. The first step in indole mineralization was the formation of a hydroxylated intermediate, oxindole. The rate of transformation of indole to oxindole and its subsequent disappearance was dependent on the concentration of inoculum and indole and the incubation temperature. Methanogenesis appeared to be the dominant process in the mineralization of indole in 10% digested sludge even in the presence of high concentrations of sulfate. Enrichment of the digested sludge with sulfate as an electron acceptor allowed the isolation of a metabolically stable mixed culture of anaerobic bacteria which transformed indole to oxindole and acetate, and ultimately to methane and carbon dioxide. This mixed culture exhibited a predominance of sulfate-reducers over methanogens with more than 75% of the substrate mineralized to carbon dioxide. The investigation demonstrates that indole can be transformed by both methanogenic and sulfate-reducing microbial populations. Springer-Verlag New York Inc., 1989 Shanker Rishi Laboratory of Soil Biochemistry, The Pennsylvania State University, 16802, University Park, Pennsylvania, U.S.A. aut Bollag Jean-Marc Laboratory of Soil Biochemistry, The Pennsylvania State University, 16802, University Park, Pennsylvania, U.S.A. aut Microbial Ecology Springer-Verlag 20/1(1990-12-01), 171-183 0095-3628 20:1<171 1990 20 248 https://doi.org/10.1007/BF02543875 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02543875 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shanker Rishi Laboratory of Soil Biochemistry, The Pennsylvania State University, 16802, University Park, Pennsylvania, U.S.A aut NATIONALLICENCE 700 1- Bollag Jean-Marc Laboratory of Soil Biochemistry, The Pennsylvania State University, 16802, University Park, Pennsylvania, U.S.A aut NATIONALLICENCE 773 0- Microbial Ecology Springer-Verlag 20/1(1990-12-01), 171-183 0095-3628 20:1<171 1990 20 248 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer