caa a22 4500 465790712 CHVBK 20180323112023.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF02543863 doi (NATIONALLICENCE)springer-10.1007/BF02543863 Persistence of genetically engineered Erwinia carotovora in perturbed and unperturbed aquatic microcosms and effect on recovery of indigenous bacteria [Elektronische Daten] [Vjera Scanferlato, George Lacy, John Cairns Jr.] Genetically engineeredErwinia carotovora persisted significantly longer in thermally perturbed microcosms (35 days) than in nonstressed microcosms (5 days). Decreased pressure of competitors and predators and increased nutrient availability were examined as the most probable reasons for greater vulnerability of perturbed microcosms to colonization by genetically engineered microorganisms (GEMs). Indigenous bacteria that competed with GEMs for the same nutrient sources (protein, cellulose, pectate) were present immediately after perturbation in densities one to two orders of magnitude lower than in unperturbed microcosms, but their populations increased to densities significantly higher than in unperturbed microscosms 10 to 15 days after inoculation. Predators of bacteria (protozoans, cladocerans, nematodes, and rotifers) were present during the experiment in unperturbed microcosms, while dense populations of bacteriovorous nanoflagellates developed in perturbed microcosms. Preemptive inoculation of perturbed microcosms with GEMs did not have a longlasting effect on the recovery of total, proteolytic, cellulolytic, and pectolytic bacteria in perturbed microscosms, indicating the absence of competitive exclusion. Springer-Verlag New York Inc., 1989 Scanferlato Vjera University Center for Environmental and Hazardous Materials Studies, Virginia Polytechnic Institute and State University, 24061, Blacksburg, Virginia, USA aut Lacy George University Center for Environmental and Hazardous Materials Studies, Virginia Polytechnic Institute and State University, 24061, Blacksburg, Virginia, USA aut Cairns Jr. John University Center for Environmental and Hazardous Materials Studies, Virginia Polytechnic Institute and State University, 24061, Blacksburg, Virginia, USA aut Microbial Ecology Springer-Verlag 20/1(1990-12-01), 11-20 0095-3628 20:1<11 1990 20 248 https://doi.org/10.1007/BF02543863 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02543863 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Scanferlato Vjera University Center for Environmental and Hazardous Materials Studies, Virginia Polytechnic Institute and State University, 24061, Blacksburg, Virginia, USA aut NATIONALLICENCE 700 1- Lacy George University Center for Environmental and Hazardous Materials Studies, Virginia Polytechnic Institute and State University, 24061, Blacksburg, Virginia, USA aut NATIONALLICENCE 700 1- Cairns Jr John University Center for Environmental and Hazardous Materials Studies, Virginia Polytechnic Institute and State University, 24061, Blacksburg, Virginia, USA aut NATIONALLICENCE 773 0- Microbial Ecology Springer-Verlag 20/1(1990-12-01), 11-20 0095-3628 20:1<11 1990 20 248 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer