caa a22 4500 465795072 CHVBK 20180323112035.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00308567 doi (NATIONALLICENCE)springer-10.1007/BF00308567 The effect of peptides containing the arginine-glycine-aspartic acid sequence on the adsorption of foot-and-mouth disease virus to tissue culture cells [Elektronische Daten] [Barry Baxt, Yechiel Becker] Sequencing of the VP1 of a large number of subtypes of foot-and-mouth disease virus (FMDV) has revealed the presence of a conserved arginine-glycine-aspartic acid (RGD) sequence located in a highly exposed region. This sequence has been shown to be essential for the interaction of certain extracellular matrix and adhesion proteins with a superfamily of cell-surface receptors called integrins. We have examined the effects of synthetic peptides containing the RGD sequence on the binding of eight different subtypes of FMDV to tissue culture cells. The results showed that such peptides inhibited viral adsorption by 50-80%. The inhibition was dose dependent but not as great as that achieved by using a saturating amount of virus as an inhibitor. Substitution of other amino acids for any of the three main residues lowered the inhibitory properties of the peptides. These results suggest that the RGD sequence in FMDV VP1 appears to be important for the interaction of virus with cellular receptor sites. Kluwer Academic Publishers, 1990 FMDV nationallicence RGD sequence nationallicence integrins nationallicence viral adsorption nationallicence synthetic peptides nationallicence Baxt Barry Molecular Biology Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, USA aut Becker Yechiel Department of Molecular Virology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel aut Virus Genes Kluwer Academic Publishers 4/1(1990-06-01), 73-83 0920-8569 4:1<73 1990 4 11262 https://doi.org/10.1007/BF00308567 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00308567 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baxt Barry Molecular Biology Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, USA aut NATIONALLICENCE 700 1- Becker Yechiel Department of Molecular Virology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/1(1990-06-01), 73-83 0920-8569 4:1<73 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer