caa a22 4500 465795110 CHVBK 20180323112035.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00308564 doi (NATIONALLICENCE)springer-10.1007/BF00308564 In-vitro translation of cucumoviral satellites III. Translational efficiencies of cucumber mosaic virus-associated RNA 5 sequence variants can be related to the predicted secondary structures of their first 55 nucleotides [Elektronische Daten] [M. Steen, J. Kaper, C. Pleij, J. Hansen] The cucumber mosaic virus (CMV) satellites D- and S-CARNA 5 (CARNA 5= Cucumber mosaic virus-Associated RNA 5), their full-length cDNA clone transcripts, and DNA clone transcripts of their open reading frames (ORFs) were used as mRNAs in the wheat-germ in-vitro translation system. Natural D-CARNA 5 yielded an anomalously large polypeptide, while transcripts made from cDNA clones of D-CARNA 5 or its first ORF had no mRNA activity. Transcripts made from the second major ORF in D-CARNA 5 yielded a smaller product, consistent with its size. Natural S-CARNA 5 and its cDNA clone transcripts both yielded the two polypeptides previously reported, while transcripts of its only major ORF yielded exclusively the smaller of the two products. The potential for an alternate initiation codon, 36 nucleotides upstream, being the source of the larger of the two polypeptides was tested. The differences in the translational properties of D- and S-CARNA 5 were related to the predicted secondary structures of the first 55 nucleotides in these CARNA 5 sequence variants. The calculated free energies of the predicted hairpins correlated inversely with their in-vitro translational activities. Kluwer Academic Publishers, 1990 in vitro translation nationallicence CARNA 5 nationallicence secondary structure nationallicence translation initiation nationallicence Steen M. Microbiology and Plant Pathology Laboratory, Plant Sciences Institute, A.R.S., U.S.D.A., Beltsville, MD, U.S.A. aut Kaper J. Microbiology and Plant Pathology Laboratory, Plant Sciences Institute, A.R.S., U.S.D.A., Beltsville, MD, U.S.A. aut Pleij C. Department of Biochemistry, State University of Leiden, Leiden, The Netherlands aut Hansen J. Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, U.S.A. aut Virus Genes Kluwer Academic Publishers 4/1(1990-06-01), 41-52 0920-8569 4:1<41 1990 4 11262 https://doi.org/10.1007/BF00308564 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00308564 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Steen M. Microbiology and Plant Pathology Laboratory, Plant Sciences Institute, A.R.S., U.S.D.A., Beltsville, MD, U.S.A aut NATIONALLICENCE 700 1- Kaper J. Microbiology and Plant Pathology Laboratory, Plant Sciences Institute, A.R.S., U.S.D.A., Beltsville, MD, U.S.A aut NATIONALLICENCE 700 1- Pleij C. Department of Biochemistry, State University of Leiden, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Hansen J. Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, U.S.A aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/1(1990-06-01), 41-52 0920-8569 4:1<41 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer