caa a22 4500 465795137 CHVBK 20180323112035.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00308565 doi (NATIONALLICENCE)springer-10.1007/BF00308565 Physical mapping of two temperature-sensitive adenovirus mutants affected in the DNA polymerase and DNA binding protein [Elektronische Daten] [Dick Roovers, C. Young, Hans Vos, John Sussenbach] We have determined the exact nature of two thermosensitive (ts) adenovirus mutants, H5ts19 and H5ts149, which map to different genes in the E2 transcription unit. The H5ts19 mutation appears to stem from a single base-pair change of A-T to G-C at position 1840 (numbering as in ref. 1), corresponding to codon 154 of the gene coding for DBP. This results in a glutamine-to-arginine change in the amino-terminal domain of the protein. H5ts19 is defective in a late stage of infection, during virus assembly. This phenotype strongly differs from that described for the limited number of known DBP mutants, indicating that DBP is not only functional during DNA replication, but also plays a role in the late phase of the infection cycle. Kluwer Academic Publishers, 1990 adenovirus nationallicence mutants nationallicence DNA replication nationallicence DNA polymerase nationallicence DNA binding protein nationallicence Roovers Dick Laboratory for Physiological Chemistry, State University of Utrecht, Utrecht, The Netherlands aut Young C. Department of Microbiology, Columbia University, College of Physicians and Surgeons, New York, NY, USA aut Vos Hans Laboratory for Physiological Chemistry, State University of Utrecht, Utrecht, The Netherlands aut Sussenbach John Laboratory for Physiological Chemistry, State University of Utrecht, Utrecht, The Netherlands aut Virus Genes Kluwer Academic Publishers 4/1(1990-06-01), 53-61 0920-8569 4:1<53 1990 4 11262 https://doi.org/10.1007/BF00308565 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00308565 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Roovers Dick Laboratory for Physiological Chemistry, State University of Utrecht, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Young C. Department of Microbiology, Columbia University, College of Physicians and Surgeons, New York, NY, USA aut NATIONALLICENCE 700 1- Vos Hans Laboratory for Physiological Chemistry, State University of Utrecht, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Sussenbach John Laboratory for Physiological Chemistry, State University of Utrecht, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Virus Genes Kluwer Academic Publishers 4/1(1990-06-01), 53-61 0920-8569 4:1<53 1990 4 11262 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer